Testis specific serine/threonine kinase 4 (Tssk4) maintains its kinase activity by phosphorylating itself at Thr-197
Testis specific serine/threonine kinase 4 (Tssk4) maintains its kinase activity by phosphorylating itself at Thr-197
复制标题
睾丸特异性丝氨酸/苏氨酸激酶 4 (Tssk4) 通过在 Thr-197 处磷酸化自身来维持其激酶活性
作者：
You;Xiaoli Wang;G. Fu;Long Yu
睾丸特异性丝氨酸/苏氨酸蛋白激酶家族（Tssk）成员在精子发生和/或精子形成中起重要作用。与其他Tssk家族成员相似，Tssk 4蛋白在睾丸中特异表达，但其生化和生物学功能尚不清楚。在目前的工作中，我们产生了一个多克隆抗体，它特异性地识别Tssk 4，但不是其他三个Tssk家族成员（Tssk 1，Tssk 2和Tssk 3）。通过使用合格的抗体，我们发现Tssk 4蛋白在从单倍体圆形精子细胞到形态成熟精子的睾丸中持续表达。进一步的体外实验表明，Tssk 4具有自磷酸化活性和自缔合特性。重要的是，我们发现Tssk 4在T环区Thr-197的自磷酸化对其激酶活性至关重要。综上所述，这些发现表明，在Thr-197的自磷酸化在维持Tssk 4激酶活性中起着关键作用，这可能参与精子发生。
The testis specific serine/threonine protein kinase family (Tssk) members play important roles in spermatogenesis and/or spermiogenesis. Similar to other Tssk family members, Tssk4 protein shows exclusive expression in testis, but its biochemical and biological functions are still largely unknown. In present work, we generate a polyclonal antibody which specifically recognizes Tssk4 but not the other three Tssk family members (Tssk1, Tssk2 and Tssk3). By using the qualified antibody, we show that Tssk4 protein is constantly expressed in testis from haploid round spermatids to morphological mature spermatozoa. Further experiments reveal that Tssk4 has autophosphorylation activity and self-association character in vitro. Importantly, we find that autophosphorylation of Tssk4 at Thr-197 in the T-loop region is essential to its kinase activity. Taken together, these findings suggest that autophosphorylation at Thr-197 plays a critical role in maintaining Tssk4 kinase activity, which might be involved in spermiogenesis.
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DOI：
--
发表时间：
2000
期刊：
--
影响因子：
--
作者：
D. Ahn;I. Ruvinsky;Andrew C. Oates;Lee M. Silver;Robert K. Ho
通讯作者：
D. Ahn;I. Ruvinsky;Andrew C. Oates;Lee M. Silver;Robert K. Ho
影响因子：
4
作者：
Li, Yahui;Sosnik, Julian;Salicioni, Ana M.
通讯作者：
Salicioni, Ana M.
影响因子：
4
作者：
Hao, ZL;Jha, KN;Visconti, PE
通讯作者：
Visconti, PE
影响因子：
2.7
作者：
Xu, Bingfang;Hao, Zhonglin;Herr, John C.
通讯作者：
Herr, John C.