3' UTRs are the primary regulators of gene expression in the C. elegans germline.
3' UTRs are the primary regulators of gene expression in the C. elegans germline.
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DOI：
10.1016/j.cub.2008.08.013
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发表时间：
2008-10-14
期刊：
影响因子：
9.2
通讯作者：
Seydoux, Geraldine
中科院分区：
文献类型：
--
作者：
Merritt, Christopher;Rasoloson, Dominique;Ko, Darae;Seydoux, Geraldine
基因是如何被调节来为每一种细胞类型产生正确的蛋白质组合是生物学中的一个基本问题。对许多基因来说，调控始于DNA水平，使用启动子序列来控制转录。转录后也可以利用mRNA的3 '非翻译区（UTR）中的序列进行调控，从而影响mRNA的稳定性和/或翻译。利用转基因实验，我们比较了启动子和3'UTRs对秀丽隐杆线虫种系基因调控的贡献。我们发现，对于大多数被测试的基因，3 ' utr是足够的调控。除了在精子发生过程中激活的启动子外，启动子在所有生殖细胞类型（从未分化的祖细胞到成熟的卵母细胞和精子）中都可以表达，并且没有细胞类型特异性。在祖细胞中，3 ' utr通过涉及两类rna结合蛋白（PUF和KH结构域蛋白）的转录后机制抑制减数分裂和卵母细胞蛋白的产生。我们的研究结果表明，在种系发育过程中，许多基因主要依靠3 ' utr而不是启动子进行调控。
How genes are regulated to produce the correct assortment of proteins for every cell type is a fundamental question in biology. For many genes, regulation begins at the DNA level using promoter sequences to control transcription. Regulation can also occur after transcription using sequences in the 3’ untranslated region (UTR) of the mRNA to affect mRNA stability and/or translation. Using a transgenic assay, we have compared the contribution of promoters and 3’UTRs to gene regulation in the C. elegans germline. We find that for most genes tested, 3’ UTRs were sufficient for regulation. With the exception of promoters activated during spermatogenesis, promoters were permissive for expression in all germ cell types (from undifferentiated progenitors to mature oocytes and sperm) and showed no cell-type specificity. In progenitors, 3’ UTRs inhibit the production of meiotic and oocyte proteins by post-transcriptional mechanisms involving two classes of RNA-binding proteins (PUF and KH domain proteins). Our findings indicate that many genes rely primarily on 3’UTRs, not promoters, for regulation during germline development.
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影响因子：
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作者：
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影响因子：
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DOI：
10.1242/dev.02490
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期刊：
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