Posthemorrhagic shock mesenteric lymph primes circulating neutrophils and provokes lung injury.
Posthemorrhagic shock mesenteric lymph primes circulating neutrophils and provokes lung injury.
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失血后休克肠系膜淋巴启动循环中性粒细胞并引起肺损伤。
DOI：
10.1006/jsre.1999.5569
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Silliman,CC
中科院分区：
文献类型：
--
作者：
Zallen,G;Moore,EE;Johnson,JL;Tamura,DY;Ciesla,DJ;Silliman,CC
肠系膜淋巴最近被用作肠源性因子的途径，这些因子可能导致失血性休克后的远端器官损伤。我们证实了失血性休克后肠系膜淋巴启动中性粒细胞(PMN)并导致肺损伤。方法从失血性休克前、中、后的肠系膜淋巴管收集肠系膜淋巴(MAP 40，持续90分钟)。然后用失血加乳酸林格氏液(2倍失血)复苏大鼠3h，通过向肺泡灌洗伊文氏蓝染色法评估肺渗漏。分离的人中性粒细胞分别与1和10%的淋巴孵育，通过流式细胞仪检测mLP(1μM)刺激的超氧化物歧化酶的产生和CD11b的表面表达。结果复苏过程中肠系膜淋巴流量显著增加：休克前144.4μL/h，休克44.5μL/h，复苏566.6μL/h，且与未转流的大鼠相比，转流淋巴可减少肺损伤。最后，休克后动物的肠系膜淋巴刺激PMN产生超氧化物(几乎是对照细胞的3倍)，并增加CD11b的表面表达(是对照的2倍)。结论肠系膜淋巴刺激PMN并导致失血性休克后肺损伤。肠系膜淋巴为促炎介质提供了管道，这些介质可能参与MOF的发病机制。
Mesenteric lymph has recently been invoked as an avenue for gut-derived factors that may result in distant organ injury following hemorrhagic shock. We demonstrate that posthemorrhagic shock mesenteric lymph primes neutrophils (PMNs) and causes lung injury.MethodsMesenteric lymph was collected from Sprague-Dawley rats from their mesenteric lymph duct prior to, during, and following hemorrhagic shock (MAP 40 for 90 min). The rats were then resuscitated with shed blood plus lactated Ringers (2X shed blood) over 3 h. Lung leak was assessed by transudation of Evan's blue dye into the alveolus as measured by bronchoalveolar lavage. Isolated human PMNs were incubated with 1 and 10% lymph; priming was measured by the fMLP (1 μM)-stimulated production of superoxide and surface expression of CD11b determined by flow cytometry.ResultsMesenteric lymph flow increased significantly during resuscitation: preshock 144.4 μl/h, shock 44.5 μl/h, resuscitation 566.6 μl/h. Furthermore, diversion of this lymph abrogated lung injury as compared to rats without lymph diversion. Finally, mesenteric lymph from postshock animals primed PMNs for superoxide production (nearly three times control cells) as well as increased surface expression of CD11b (2-fold over control).ConclusionMesenteric lymph primes PMNs and causes lung injury following hemorrhagic shock. Mesenteric lymph provides a conduit for proinflammatory mediators that may participate in the pathogenesis of MOF.
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肠道缺血中的内毒素转运途径和动力学。
DOI：
--
发表时间：
1985
期刊：
Acta chirurgica Scandinavica
影响因子：
--
作者：
P. Olofsson;G. Nylander;P. Olsson
通讯作者：
P. Olsson
DOI：
10.1097/00005373-198807000-00002
发表时间：
1988-07-01
影响因子：
--
作者：
BAKER, JW;DEITCH, EA;SPECIAN, RD
通讯作者：
SPECIAN, RD
血小板激活因子引发中性粒细胞对激动剂的反应：在促进中性粒细胞介导的内皮损伤中的作用。
影响因子：
20.3
作者：
Vercellotti,GM;Yin,HQ;Gustafson,KS;Nelson,RD;Jacob,HS
通讯作者：
Jacob,HS
影响因子：
3.1
作者：
MAINOUS, MR;ERTEL, W;DEITCH, EA
通讯作者：
DEITCH, EA
影响因子：
2.2
作者：
KOIKE, K;MOORE, FA;BANERJEE, A
通讯作者：
BANERJEE, A