A Novel Mouse Model of Liver Ischemic/Reperfusion Injury and its Differences to the Existing Model
A Novel Mouse Model of Liver Ischemic/Reperfusion Injury and its Differences to the Existing Model
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一种新型小鼠肝脏缺血/再灌注损伤模型及其与现有模型的差异
DOI：
10.3109/08941939.2014.983621
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发表时间：
2015-07
影响因子：
1.9
通讯作者：
Qiang Xia
中科院分区：
文献类型：
--
作者：
Jianjian Zhang;Ming Zhang;Jianjun Zhang;Qiang Xia
摘要背景：头叶缺血(占肝脏质量的70%)是一种常用的小鼠肝脏缺血/再灌注(I/R)模型，不涉及流出道阻断。该模型产生的结果具有相对较大的方差。材料和方法：建立一种新的左外叶(占肝脏质量的35%)缺血模型，包括暂时阻断头叶的血供以排出血液，然后阻断左外叶的流入和流出。35%(新型)和70%(现有)模型组小鼠造成I/R损伤，并对血液和肝脏标本进行生化和组织学分析。同时测定缺血叶的组织氧分压(TPO2)以确定肝组织是否处于稳定的低氧状态。对生化结果、组织学评分和tPO2水平进行统计分析，并计算变异系数(CV)。结果：35%组的谷草转氨酶活性、组织学评分和tPO2水平的变异系数明显低于70%组。TPO2测量显示，70%模型的血流阻断并未导致稳定的低氧状态，即使在门静脉三联结扎和切断后也是如此，这表明存在来自下腔静脉的血液回流，这可能是70%I/R模型结果差异的原因。结论：新的35%I/R模型的结果是可重复性的，因为缺血叶的流入和流出都被阻断了。
ABSTRACT Background: Ischemia of the cephalad lobes (70% of liver mass) is a frequently employed mouse hepatic ischemia/reperfusion (I/R) model that does not involve outflow occlusion. This model produces results with relatively large variances. Materials and Methods: A novel model of ischemia of the left lateral lobe (35% of liver mass) that involves temporarily occluding the blood supply to the cephalad lobes to expel blood followed by occlusion of both the inflow and outflow of the left lateral lobe, was developed. Mice in the 35% (novel) and 70% (existing) model groups were subjected to I/R injury, and biochemical and histological analyses of blood and liver samples were performed. Tissue oxygen partial pressure (tPO2) measurements in the ischemic lobes were also performed to determine whether the hepatic tissue was in a stable hypoxic state. Statistical analyses of the biochemical results, histological scores, and tPO2 levels were performed from which coefficients of variation (CV) were calculated. Results: The CVs of the aminotransferase activities, histological scores, and tPO2 levels were much lower in the 35% group than those in the 70% group. The tPO2 measurements demonstrated that inflow occlusion in the 70% model did not result in a stable hypoxic state, even after the portal triads were ligated and severed, indicating that there was blood reflux from the vena cava, which would be responsible for the variations in results with the 70% I/R model. Conclusions: The new 35% I/R model leads to reproducible results because both inflow and outflow of the ischemic lobe are occluded.
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影响因子：
20.3
作者：
Tepper, OM;Capla, JM;Gurtner, GC
通讯作者：
Gurtner, GC
DOI：
10.1620/tjem.138.63
发表时间：
1982-09
期刊：
The Tohoku journal of experimental medicine
影响因子：
--
作者：
H. Yamauchi;I. Baća;U. Mittmann;H. Geisen;M. Salzer
通讯作者：
H. Yamauchi;I. Baća;U. Mittmann;H. Geisen;M. Salzer
DOI：
--
发表时间：
1982
期刊：
Acta chirurgica Scandinavica
影响因子：
--
作者：
P. Hasselgren;E. Jennische;J. Fornander;A. Hellman
通讯作者：
P. Hasselgren;E. Jennische;J. Fornander;A. Hellman
影响因子：
13.5
作者：
Yadav, SS;Sindram, D;Clavien, PA
通讯作者：
Clavien, PA
影响因子：
13.5
作者：
Hamada, Takashi;Fondevila, Constantino;Coito, Ana J.
通讯作者：
Coito, Ana J.