Interplay of a non-conjugative integrative element and a conjugative plasmid in the spread of antibiotic resistance via suicidal plasmid transfer from an aquaculture Vibrio isolate.
Interplay of a non-conjugative integrative element and a conjugative plasmid in the spread of antibiotic resistance via suicidal plasmid transfer from an aquaculture Vibrio isolate.
复制标题
DOI：
10.1371/journal.pone.0198613
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Yano H
中科院分区：
文献类型：
--
作者：
Nonaka L;Yamamoto T;Maruyama F;Hirose Y;Onishi Y;Kobayashi T;Suzuki S;Nomura N;Masuda M;Yano H
移动的遗传元件（MGE）捕获抗菌素耐药基因（ARG）在人类相关细菌的耐药性获得中起着关键作用。虽然水产养殖环境被认为是ARGs的重要水库，但在海洋生物中发现的MGE的细胞内和细胞间流动性特征不佳。在这里，我们展示了一种新的模式，种间ARGs转移涉及一个“非共轭”的整合元素。为了确定活跃的MGE在弧菌分离，我们进行了全基因组测序的接合子之间的大肠杆菌和弧菌ponticus交配。这揭示了质粒（命名为pSEA 1）整合到染色体中，该质粒由MOBH组的自传递质粒骨架ARG和13.8-kb整合元件Tn 6283组成。分子遗传学分析表明，该基因转移模式为两步式。首先，Tn 6283在自杀性质粒转移过程中整合到受体染色体中，随后染色体中的Tn 6283拷贝与新转移的pSEA 1中的拷贝之间发生同源重组。Tn 6283在整合元件中是不寻常的，因为它显然不编码转移功能，并且它的切除几乎不产生未被占据的供体位点。因此，它的运动类似于插入序列的转座，而不是典型的整合和共轭元件的转座。总体而言，这项研究揭示了海洋生物中存在一种以前未被识别的MGE类型，突出了种间基因转移模式的多样性。
The capture of antimicrobial resistance genes (ARGs) by mobile genetic elements (MGEs) plays a critical role in resistance acquisition for human-associated bacteria. Although aquaculture environments are recognized as important reservoirs of ARGs, intra- and intercellular mobility of MGEs discovered in marine organisms is poorly characterized. Here, we show a new pattern of interspecies ARGs transfer involving a ‘non-conjugative’ integrative element. To identify active MGEs in a Vibrio ponticus isolate, we conducted whole-genome sequencing of a transconjugant obtained by mating between Escherichia coli and Vibrio ponticus. This revealed integration of a plasmid (designated pSEA1) into the chromosome, consisting of a self-transmissible plasmid backbone of the MOBH group, ARGs, and a 13.8-kb integrative element Tn6283. Molecular genetics analysis suggested a two-step gene transfer model. First, Tn6283 integrates into the recipient chromosome during suicidal plasmid transfer, followed by homologous recombination between the Tn6283 copy in the chromosome and that in the newly transferred pSEA1. Tn6283 is unusual among integrative elements in that it apparently does not encode transfer function and its excision barely generates unoccupied donor sites. Thus, its movement is analogous to the transposition of insertion sequences rather than to that of canonical integrative and conjugative elements. Overall, this study reveals the presence of a previously unrecognized type of MGE in a marine organism, highlighting diversity in the mode of interspecies gene transfer.
登录
查看更多内容
揭示了整合静脉细菌中结合元素的普遍性和多样性。
影响因子：
3.7
作者：
Ghinet MG;Bordeleau E;Beaudin J;Brzezinski R;Roy S;Burrus V
通讯作者：
Burrus V
DOI：
10.1080/2159256x.2016.1179074
发表时间：
2016-05
期刊：
Mobile genetic elements
影响因子：
--
作者：
Harrison E;Dytham C;Hall JP;Guymer D;Spiers AJ;Paterson S;Brockhurst MA
通讯作者：
Brockhurst MA
影响因子：
11
作者：
Goudenege, David;Labreuche, Yannick;Le Roux, Frederique
通讯作者：
Le Roux, Frederique
影响因子：
48
作者：
Choi, KH;Gaynor, JB;Schweizer, HP
通讯作者：
Schweizer, HP
DOI：
10.1186/s12941-015-0100-6
发表时间：
2015-10-20
影响因子：
5.7
作者：
Deng Y;Bao X;Ji L;Chen L;Liu J;Miao J;Chen D;Bian H;Li Y;Yu G
通讯作者：
Yu G