The herpes simplex virus type 1 US7 gene product is a 66K glycoprotein and is a target for complement-dependent virus neutralization.
The herpes simplex virus type 1 US7 gene product is a 66K glycoprotein and is a target for complement-dependent virus neutralization.
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单纯疱疹病毒 1 型 US7 基因产物是一种 66K 糖蛋白，是补体依赖性病毒中和的靶标。
作者：
V. Sullivan;G. Smith
先前通过核苷酸测序鉴定的单纯疱疹病毒1型（HSV-1）的US7开放阅读框已在重组痘苗病毒（US7- vac）中表达。在us7 - vac感染的细胞中，抗HSV-1的抗血清与66K糖蛋白反应，该多肽存在于细胞核和细胞膜表面。在tunicamycin的存在下，蛋白质的大小减小到58K，表明它含有n -连接的糖残基。接种US7-VAC的动物抗血清识别HSV-1感染细胞中的66K和58K多肽，并在补体存在的情况下在体外中和HSV-1的传染性。
The US7 open reading frame of herpes simplex virus type 1 (HSV-1), previously identified by nucleotide sequencing, has been expressed in a recombinant vaccinia virus (US7-VAC). Antiserum raised against HSV-1 reacted with a 66K glycoprotein in US7-VAC-infected cells and this polypeptide was present on both nuclear and cell surface membranes. In the presence of tunicamycin the protein was reduced in size to 58K showing it contained N-linked sugar residues. Antisera from animals vaccinated with US7-VAC recognized 66K and 58K polypeptides in HSV-1-infected cells and neutralized HSV-1 infectivity in vitro in the presence of complement.
DOI：
10.1073/pnas.84.12.4303
发表时间：
1987-06
影响因子：
11.1
作者：
R. Longnecker;S. Chatterjee;R. Whitley;B. Roizman
通讯作者：
R. Longnecker;S. Chatterjee;R. Whitley;B. Roizman
通过 Tn5 诱变快速鉴定 1 型单纯疱疹病毒非必需基因。
DOI：
10.1126/science.3033824
发表时间：
1987
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Weber,PC;Levine,M;Glorioso,JC
通讯作者：
Glorioso,JC