Quantifying the impact of chemicals on stable carbon and oxygen isotope values of raw pollen

Quantifying the impact of chemicals on stable carbon and oxygen isotope values of raw pollen
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量化化学品对原始花粉稳定碳和氧同位素值的影响

DOI:
10.1002/jqs.3300
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发表时间:
2021
影响因子:
2.3
通讯作者:
Helle G.
Helle G.
中科院分区:
地球科学3区
文献类型:
--
作者:
Müller C;Hennig J;Riedel F;Helle G.

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从湖泊沉积物中提取花粉的纯化方案含有根据花粉特征和科从属关系改变碳和氧花粉同位素值的化学物质。用氢氧化钾 (KOH)、氢氟酸 (HF)、次氯酸钠 (NaClO) 和硫酸 (H2SO4) 处理阔叶树种(桤木、垂枝桦、鹅耳枥、榛子、水青冈和夏栎)和针叶树种(冷杉和樟子松)的现代(原始)花粉。测试对 δ13Cpollen 和 δ18Opollen 的影响并评估纯化方案的适用性。阔叶树和针叶树的花粉对化学暴露的反应不同,但反应模式通常相似。 δ13​​C花粉值的变化在 + 1.0‰(B. pendula,NaClO处理)和-5.0‰(P. sylvestris,H2SO4处理)之间变化。原始样品和化学处理样品的 δ13C 花粉值在用 KOH、NaClO 和 HF 处理后似乎相关,而 H2SO4 的应用导致物种间变化不一致。化学物质对 δ18Opollen 的影响更加多样化,偏移范围在 +1.1‰(C. avellana,NaClO 处理)和 -17.9‰(P. sylvestris,H2SO4 处理)之间。一般来说,在纯化方案中应尽量减少同位素改变化学品的使用,但在 δ13Cpollen 和 δ18Opollen 分析之前,KOH 和 NaClO 的应用似乎大多没有问题。
Purification protocols to extract pollen from lake sediments contain chemicals that alter the carbon and oxygen pollen‐isotope values according to pollen characteristics and family affiliation. Modern (raw) pollen of broad‐leaved (Alnus glutinosa, Betula pendula, Carpinus betulus, Corylus avellana, Fagus sylvaticaandQuercus robur) and coniferous tree species (Picea abiesandPinus sylvestris) were treated with potassium hydroxide (KOH), hydrofluoric acid (HF), sodium hypochlorite (NaClO) and sulphuric acid (H2SO4) to test the impact on δ13Cpollenand δ18Opollenand assess the applicability in purification protocols. Pollen of broad‐leaved and coniferous trees reacted differently to chemical exposure, but response patterns are generally alike. Alterations of δ13Cpollenvalues vary between + 1.0‰ (B. pendula, NaClO‐treatment) and −5.0‰ (P. sylvestris, H2SO4‐treatment). The δ13Cpollenvalues of raw and chemically treated samples seem to be related after treatments with KOH, NaClO and HF, whereas the application of H2SO4led to inconsistent changes among species. The impact of chemicals on δ18Opollenare more diverse and offsets range between +1.1‰ (C. avellana, NaClO‐treatment) and −17.9‰ (P. sylvestris, H2SO4‐treatment). In general, the use of isotope‐altering chemicals in purification protocols should be brought to a minimum, but the application of KOH and NaClO seems mostly unproblematic before δ13Cpollenand δ18Opollenanalysis.
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