Quantitative and functional posttranslational modification proteomics reveals that TREPH1 plays a role in plant touch-delayed bolting
Quantitative and functional posttranslational modification proteomics reveals that TREPH1 plays a role in plant touch-delayed bolting
复制标题
定量和功能翻译后修饰蛋白质组学揭示 TREPH1 在植物接触延迟抽苔中发挥作用
DOI：
10.1073/pnas.1814006115
复制
发表时间：
2018-08
影响因子：
11.1
通讯作者：
Li Ning
中科院分区：
文献类型：
--
作者：
Wang Kai;Yang Zhu;Qing Dongjin;Ren Feng;Liu Shichang;Zheng Qingsong;Liu Jun;Zhang Weiping;Dai Chen;Wu Madeline;Chehab E Wassim;Braam Janet;Li Ning
意义 植物对微妙的力信号（例如轻触）做出反应，类似于动物神经系统，如触动性、触动运动和触动形态发生所证明的那样。为了了解力信号网络，我们在基于拟南芥 (SILIA) 的定量翻译后修饰蛋白质组学中应用了稳定同位素标记，以评估拟南芥中经受 40 秒棉签接触的蛋白质磷酸化变化，鉴定了 4,895 个非冗余磷酸肽，其中 579 个是先前未报告的来自 509 个磷蛋白组的磷酸位点，并鉴定了 24 个磷酸化肽。触摸调节磷酸蛋白 (TREPH) 基团。分子生物学、遗传和生物信息学分析表明，先前未表征的 TREPH1 蛋白是拟南芥触摸反应的抽苔延迟方面所必需的。这些研究表明，蛋白质磷酸化和 TREPH1 蛋白对于导致植物触动形态发生的机械转导途径至关重要。环境机械力，例如风和触摸，会触发植物中的基因表达调节和发育变化，称为“触状形态发生”，这证明了植物感知此类刺激的能力。在拟南芥中，主要的触变形态发生反应是延迟抽苔，即花茎的出现。负责触摸反应的机械转导的信号成分在很大程度上是未知的。在这里，我们进行了基于高通量 SILIA（拟南芥稳定同位素标记）的定量磷酸化蛋白质组学分析，以分析拟南芥中 40 秒的力刺激导致的蛋白质磷酸化变化。在已鉴定的 24 种触摸响应磷酸肽中，许多源自激酶、磷酸酶、细胞骨架蛋白、膜蛋白和离子转运蛋白。此外，免疫印迹证实，先前未表征的蛋白质触摸调节磷酸蛋白 1 (TREPH1) 在触摸刺激的植物中迅速磷酸化。 TREPH1 作为可溶性蛋白质进行分级，并且被证明是触摸引起的抽苔延迟和基因表达变化所必需的。此外，TREPH1 的不可磷酸化位点特异性亚型 (S625A) 未能恢复触摸诱导的 treph1-1 开花延迟，表明 S625 对 TREPH1 功能的必要性，并提供与触摸调节的 TREPH1 磷酸化可能的功能相关性一致的证据。总而言之，这些发现确定了拟南芥丝状形态发生调节中的磷蛋白参与者，并提供证据表明 TREPH1 及其触摸诱导的磷酸化可能在触摸诱导的抽苔延迟（触状形态发生的主要组成部分）中发挥作用。
Significance Plants respond to a delicate force signal, such as a light touch, similar to animal neural systems, as demonstrated by thigmotropism, thigmonastic movement, and thigmomorphogenesis. To understand the force-signaling networks, we applied stable isotope labeling in Arabidopsis (SILIA)-based quantitative posttranslational modification proteomics to assess protein phosphorylation changes in Arabidopsis subjected to 40-second cotton-swab touch, identified 4,895 nonredundant phosphopeptides, 579 of which are previously unreported phosphosites derived from 509 phosphoprotein groups, and identified 24 TOUCH-REGULATED PHOSPHOPROTEIN (TREPH) groups. Molecular biological, genetic, and bioinformatic analyses revealed that the previously uncharacterized TREPH1 protein is required for the bolting-delay aspect of the Arabidopsis touch response. These studies suggest that protein phosphorylation and the TREPH1 protein are critical for the mechanotransduction pathway leading to an aspect of plant thigmomorphogenesis. Environmental mechanical forces, such as wind and touch, trigger gene-expression regulation and developmental changes, called “thigmomorphogenesis,” in plants, demonstrating the ability of plants to perceive such stimuli. In Arabidopsis, a major thigmomorphogenetic response is delayed bolting, i.e., emergence of the flowering stem. The signaling components responsible for mechanotransduction of the touch response are largely unknown. Here, we performed a high-throughput SILIA (stable isotope labeling in Arabidopsis)-based quantitative phosphoproteomics analysis to profile changes in protein phosphorylation resulting from 40 seconds of force stimulation in Arabidopsis thaliana. Of the 24 touch-responsive phosphopeptides identified, many were derived from kinases, phosphatases, cytoskeleton proteins, membrane proteins, and ion transporters. In addition, the previously uncharacterized protein TOUCH-REGULATED PHOSPHOPROTEIN1 (TREPH1) became rapidly phosphorylated in touch-stimulated plants, as confirmed by immunoblots. TREPH1 fractionates as a soluble protein and is shown to be required for the touch-induced delay of bolting and gene-expression changes. Furthermore, a nonphosphorylatable site-specific isoform of TREPH1 (S625A) failed to restore touch-induced flowering delay of treph1-1, indicating the necessity of S625 for TREPH1 function and providing evidence consistent with the possible functional relevance of the touch-regulated TREPH1 phosphorylation. Taken together, these findings identify a phosphoprotein player in Arabidopsis thigmomorphogenesis regulation and provide evidence that TREPH1 and its touch-induced phosphorylation may play a role in touch-induced bolting delay, a major component of thigmomorphogenesis.
登录
查看更多内容
光和重力信号在调节植物的发展方面协同作用。
影响因子：
5.6
作者：
Vandenbrink JP;Kiss JZ;Herranz R;Medina FJ
通讯作者：
Medina FJ
影响因子：
7.4
作者：
Lin Zhu;Dandan Liu;Yaojun Li;Ning Li
通讯作者：
Lin Zhu;Dandan Liu;Yaojun Li;Ning Li
影响因子：
23.9
作者：
Hamilton ES;Schlegel AM;Haswell ES
通讯作者：
Haswell ES
影响因子：
16.6
作者：
Cleverley RM;Barrett JR;Baslé A;Bui NK;Hewitt L;Solovyova A;Xu ZQ;Daniel RA;Dixon NE;Harry EJ;Oakley AJ;Vollmer W;Lewis RJ
通讯作者：
Lewis RJ
影响因子：
64.8
作者：
L. Tait
通讯作者：
L. Tait