Effect of dietary Lactobacilli mixture on Listeria monocytogenes infection and virulence property in broilers.
Effect of dietary Lactobacilli mixture on Listeria monocytogenes infection and virulence property in broilers.
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DOI：
10.1016/j.psj.2020.03.058
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发表时间：
2020-07
期刊：
影响因子：
4.4
通讯作者：
Liu N
中科院分区：
文献类型：
--
作者：
Deng Q;Shi H;Luo Y;Zhao H;Liu N
本研究旨在探讨益生菌乳酸杆菌对1 ~ 14日龄肉仔鸡单核细胞增生李斯特菌负荷、炎症反应和毒力特性的影响。试验选用480只肉仔鸡，随机分为4个处理，每个处理6个重复。所有鸡均感染L.在第一天添加单核细胞增多症，并以0（对照）、106、108、1010 cfu/kg饮食的剂量补充等量的嗜酸乳杆菌和植物乳杆菌的混合物。结果表明，在给药后第7天和第14天，添加3种剂量的乳酸杆菌可使盲肠、皮肤、肝脏和脾脏中的单核细胞增生李斯特菌菌负荷降低（P < 0.05）0.065 ~ 0.933 log 10 cfu，且皮肤中的致病菌随益生菌剂量的增加呈线性下降（P ≤ 0.015）。益生菌治疗组血清IL-1β、IL-6、肿瘤坏死因子-α和干扰素-γ等细胞因子水平降低25.4 ~ 51.1%（P < 0.05）。乳酸杆菌处理组肠粘膜中与抗炎反应相关的基因包括IL-10、缺氧诱导因子1 α（HIF 1A）、前列腺素E受体2和前列腺素内过氧化物合成酶2的转录水平上调（P < 0.05），HIF 1A的线性和二次响应（P ≤ 0.019）。此外，益生菌通过下调自溶素Ami、枯草芽孢杆菌溶血素O、内化素A和B来减弱（P < 0.05）鞭毛粘附、孔形成和侵袭特性，并且益生菌对鞭毛蛋白表现出线性（P = 0.006）剂量反应。研究结果表明，饮食中同时给予L。嗜酸乳杆菌和L. plantarum能使L.结果表明，益生菌制剂可通过抑制单核细胞增多症病毒的肠道接种、移位、炎症反应和毒力等途径抑制单核细胞增多症病毒感染，可作为预防肉鸡单核细胞增多症病毒感染的替代制剂。
The present study aimed to investigate the effect of probiotic Lactobacilli addition on Listeria monocytogenes load, inflammatory reaction, and virulence properties in broilers from 1 to 14 D of age. A total of 480 broiler chicks were randomly allocated to 4 treatments of 6 replicates each. All birds were infected with L. monocytogenes on the first day and supplemented an equal amount mixture of Lactobacillus acidophilus and Lactobacillus plantarum at doses of 0 (control), 106, 108, 1010 cfu/kg of diet. The results showed that on 7 and 14 D after administration, Lactobacilli addition at the 3 doses decreased (P < 0.05) L. monocytogenes loads in the cecum, skin, liver, and spleen by 0.065 to 0.933 log10 cfu, and the pathogen linearly reduced (P ≤ 0.015) with the increasing doses of probiotics in the skin. Serum cytokines including IL-1β, IL-6, tumor necrosis factor-α, and interferon-γ in probiotics treatments were decreased (P < 0.05) by 25.4 to 51.1%. Transcriptional levels of genes related to anti-inflammatory reactions including IL-10, hypoxia inducible factor 1 alpha (HIF1A), prostaglandin E receptor 2, and prostaglandin-endoperoxide synthase 2 in the intestinal mucosa were upregulated (P < 0.05) in Lactobacilli treatments, and linear and quadratic responses (P ≤ 0.019) were found on HIF1A. Furthermore, the probiotics attenuated (P < 0.05) listerial adhesion, pore-forming, and invasion properties by downregulating autolysin Ami, listeriolysin O, internalin A and B, and a linear (P = 0.006) dose response of probiotics was exhibited on flagellin. The findings indicate that dietary coadministration of L. acidophilus and L. plantarum can attenuate L. monocytogenes infection by depressing its intestinal inoculation, translocation, inflammatory reaction, and virulence property in broilers and suggest that the probiotics can be an alternative against listerial infection in broilers.
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影响因子：
30.3
作者：
Drolia R;Tenguria S;Durkes AC;Turner JR;Bhunia AK
通讯作者：
Bhunia AK
影响因子：
4.6
作者：
Novak, Sanja;Drenjancevic, Ines;Mihalj, Martina
通讯作者：
Mihalj, Martina
影响因子：
3.6
作者：
Liu, Ning;Ru, Yingjun;Xu, Tingsheng
通讯作者：
Xu, Tingsheng
影响因子：
5.2
作者：
Ayala, Diana I.;Cook, Peter W.;Nightingale, Kendra K.
通讯作者：
Nightingale, Kendra K.
DOI：
10.1016/j.ijfoodmicro.2011.06.011
发表时间：
2011-10-03
影响因子：
5.4
作者：
Moslehi-Jenabian, Saloomeh;Vogensen, Finn Kvist;Jespersen, Lene
通讯作者：
Jespersen, Lene