Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls.
Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls.
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DOI：
10.1038/s41598-020-78943-6
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发表时间：
2020-12-14
影响因子：
4.6
通讯作者：
Agner T
中科院分区：
文献类型：
--
作者：
Clausen ML;Kezic S;Olesen CM;Agner T
胶带剥离是评估炎症性皮肤病表皮生物标志物的一种很有前途的技术。然而，为了促进其在临床实践中的实施，需要对采样策略进行彻底的验证。有关生物标记物跨角质层浓度变化的知识很少。因此，本研究旨在利用胶带剥离技术对15例特应性皮炎(AD)患者和16名健康对照的皮损和非皮损皮肤连续使用21条胶带(D-Squame)，以评估细胞因子在角质层中的变化。用多重免疫法测定5个不同深度的细胞因子(IL-1α、IL-1b、IL-5、IL-18、干扰素-γ、CCL17、CCL22、CCL27、CXCL8、CXCL10、肿瘤坏死因子-α、肿瘤坏死因子-1β、肿瘤坏死因子-1β、血管内皮生长因子)的浓度。胶带4与胶带21比较，AD皮损皮肤细胞因子浓度无明显变化。在AD非皮损皮肤中，CCL17、CXCL8和CXCL10的表达略有下降。健康对照组IL-1a、IL-1b、VEGFA降低，IL-18升高。阿尔茨海默病皮肤与健康对照皮肤有差异。角质层的细胞因子浓度是稳定的，这表明只需从角质层采集一卷胶带就能可靠地反映整个细胞因子环境。AD和健康皮肤之间的差异证实了胶带剥离测量细胞因子水平的稳健性。
Tape stripping is a promising technique for assessment of epidermal biomarkers in inflammatory skin diseases. However, to facilitate its implementation in the clinical practice, a thorough validation regarding sampling strategy is needed. Knowledge of biomarkers variation in concentration across stratum corneum is scarce. Therefore, this study aimed to assess the variability of cytokines across stratum corneum using tape stripping technique by consecutive application of 21 adhesive tapes (D-squame) to lesional and non-lesional skin from 15 patients with atopic dermatitis (AD) and 16 healthy controls. Concentration of cytokines (IL-1α, IL-1b, IL-5, IL-18, IFN-γ, CCL17, CCL22, CCL27, CXCL8, CXCL10, TNF-α, TSLP, VEGFA) was determined in five different depths, using multiplex immunoassay. Comparing tape 4 with tape 21, no cytokine changed significantly in concentration in AD lesional skin. In AD non-lesional skin a small decrease was found for CCL17, CXCL8 and CXCL10. For healthy controls, a decrease was found for IL-1a, IL-1b, VEGFA and an increase for IL-18. Differences were found between AD skin and healthy control skin. Concentration of cytokines was stable across stratum corneum, indicating that sampling of only one tape from the stratum corneum is reliable in reflecting the overall cytokine milieu. Differences between AD and healthy skin confirm robustness of tape stripping for measuring cytokine levels.
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DOI：
10.1016/j.jaci.2010.12.1124
发表时间：
2011-04
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Suárez-Fariñas M;Tintle SJ;Shemer A;Chiricozzi A;Nograles K;Cardinale I;Duan S;Bowcock AM;Krueger JG;Guttman-Yassky E
通讯作者：
Guttman-Yassky E
DOI：
10.1111/bjd.17088
发表时间：
2019-03
期刊：
The British journal of dermatology
影响因子：
--
作者：
McAleer MA;Jakasa I;Hurault G;Sarvari P;McLean WHI;Tanaka RJ;Kezic S;Irvine AD
通讯作者：
Irvine AD
影响因子：
4.6
作者：
Olesen, Caroline Meyer;Fuchs, Christine Sofie Krohn;Clausen, Maja-Lisa
通讯作者：
Clausen, Maja-Lisa
影响因子：
6.5
作者：
Janssens, Michelle;van Smeden, Jeroen;Bouwstra, Joke A.
通讯作者：
Bouwstra, Joke A.
影响因子：
10.3
作者：
Kezic, S.;Kammeyer, A.;Bos, J. D.
通讯作者：
Bos, J. D.