Metabolic labeling of fucosylated glycoproteins in Bacteroidales species.
Metabolic labeling of fucosylated glycoproteins in Bacteroidales species.
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DOI：
10.1016/j.bmcl.2011.05.038
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Wu, Peng
中科院分区：
文献类型：
--
作者：
Besanceney-Webler, Christen;Jiang, Hao;Wang, Wei;Baughn, Anthony D.;Wu, Peng
拟杆菌目的成员是人类结肠微生物群中最丰富的革兰氏阴性细菌。这些物种用岩藻糖基化聚糖修饰其细胞表面糖蛋白，这被认为在宿主肠道定植中起重要作用。目前，还没有富集这些糖蛋白用于其鉴定的方法。在这里，我们描述了一种化学方法，直接标记和检测岩藻糖基化糖蛋白培养类杆菌属物种，即脆弱拟杆菌和Parabacteroides distasonis。我们用含炔岩藻糖类似物处理这些细菌，其代谢整合到细菌表面岩藻糖基化糖蛋白中。然后，经炔标记的糖蛋白可以通过生物正交点击化学与带有叠氮化物的生物物理探针反应，用于检测或糖蛋白质组学分析。
Members of the Bacteroidales order are among the most abundant gram-negative bacteria of the human colonic microbiota. These species decorate their cell surface glycoproteins with fucosylated glycans, which are believed to play important roles in host intestinal colonization. Currently, there is no method for the enrichment of these glycoproteins for their identification. Here, we describe a chemical approach directed toward labeling and detecting fucosylated glycoproteins from cultured Bacteroidales species, namely Bacteroides fragilis and Parabacteroides distasonis. We treated these bacteria with an alkyne-bearing fucose analog, which is metabolically integrated into the bacterial surface fucosylated glycoproteins. The alkyne-tagged glycoproteins can then react with azide-bearing biophysical probes via bioorthogonal click chemistry for detection or glycoproteomic analysis.
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.0605418103
发表时间：
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发表时间：
2002-11-26
影响因子：
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通讯作者：
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