The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.
The PI3K/Akt signaling pathway exerts effects on the implantation of mouse embryos by regulating the expression of RhoA.
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PI3K/Akt信号通路通过调节RhoA表达影响小鼠胚胎着床
DOI：
10.3892/ijmm.2014.1701
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发表时间：
2014-05
影响因子：
5.4
通讯作者：
Yu Q
中科院分区：
文献类型：
--
作者：
Liu L;Wang Y;Yu Q
本研究旨在探讨磷脂酰肌醇3-激酶(PI3K)/Akt信号通路是否通过调节RhoA的表达而影响小鼠胚胎着床。用定量逆转录聚合酶链式反应(qRT-PCR)、免疫组织化学和免疫印迹方法检测PI3K、Akt、磷酸化(p-)Akt、磷酸酶和紧张素同源物(PTEN)和RhoA在妊娠第5天(D5)小鼠子宫和假孕小鼠子宫中的表达。在妊娠第2天(D2)宫内注射PI3K抑制剂LY294002，对这些基因进行功能分析。PI3K、p-Akt、RhoA在子宫内膜种植部位的表达水平高于植入间部位，而PTEN的表达水平则相反。假孕组和注射PI3K/Akt抑制剂LY294002组上述基因的表达水平明显低于妊娠组。功能实验显示，在妊娠第2天，宫内注射PI3K抑制剂LY294002后，与对侧注射磷酸盐缓冲盐水(PBS)相比，植入点数显著减少(P<；0.05)。这些结果表明，PI3K/Akt信号通路通过调节RhoA的表达而影响胚胎着床。
The aim of this study was to investigate whether the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway affects the implantation of mouse embryos by regulating the expression of RhoA. The expression of PI3K, Akt, phosphorylated (p-)Akt, phosphatase and tensin homolog (PTEN) and RhoA in the uterus of mice on day 5 of pregnancy (D5) and in pseudopregnant mice was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot analysis. A functional analysis of these genes was also performed by the intrauterine injection with the PI3K inhibitor, LY294002, on day 2 of pregnancy (D2). The expression levels of PI3K, p-Akt, RhoA at the implantation site were higher than those at the inter-implantation site in the endometrium; however, opposite effects were observed for PTEN expression. The expression levels of the above genes in the pseudopregnant group and in the group injected with the PI3K/Akt inhibitor, LY294002, were markedly lower than those in the pregnant group. Functional experiments revealed that the number of implantation sites had been significantly decreased (P<0.05) following the intrauterine injection of the PI3K inhibitor, LY294002, on day 2 of gestation compared with the contralateral injection of phosphate-buffered saline (PBS). These results suggest that the PI3K/Akt signaling pathway affects embryo implantation by regulating the expression of RhoA.
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影响因子：
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作者：
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通讯作者：
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