Stress-Activated Protein Kinase OsSAPK9 Regulates Tolerance to Salt Stress and Resistance to Bacterial Blight in Rice
Stress-Activated Protein Kinase OsSAPK9 Regulates Tolerance to Salt Stress and Resistance to Bacterial Blight in Rice
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应激激活蛋白激酶OsSAPK9调节水稻对盐胁迫的耐受性和对白叶枯病的抵抗力
DOI：
10.1186/s12284-019-0338-2
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发表时间：
2019-11
期刊：
影响因子：
5.5
通讯作者：
Yongli Zhou
中科院分区：
文献类型：
--
作者：
Fan Zhang;Dan Zeng;Liyu Huang;Yingyao Shi;Tengjun Chen;Fan Zhang;Yongli Zhou
盐胁迫与水稻黄单胞菌引起的细菌性枯萎病。稻瘟病菌（Xoo）是水稻（Oryza sativaL）产量的关键限制因子。蔗糖非发酵1 （SNF1）相关蛋白激酶2 （SnRK2）是植物特异性丝氨酸/苏氨酸激酶家族的成员，是参与植物发育过程和逆境响应的信号通路的重要组成部分。水稻中SnRK2家族有10个成员；然而，人们对它们的功能和潜在的分子机制知之甚少。结果本研究发现，隶属SnRK2家族的ossapk9正调控水稻的盐胁迫耐受性和对白叶枯病的抗性。RNA测序结果显示，与野生型植物相比，盐胁迫条件下和接种后ossapk9 - rnaiin中分别有404和1324个基因表达差异，这些基因参与基本代谢过程。在盐胁迫条件下和接种后，共检测到65个主要参与防御反应的常见差异表达基因。此外，体内和体外实验表明，OsSAPK9与分子伴侣OsSGT1和OsHsp90形成蛋白复合物，过表达gossgt1的转基因植株对盐胁迫的耐受性降低，对白叶枯病的抗性水平显著提高。因此，OsSAPK9可能通过与OsSGT1的相互作用，在水稻盐胁迫反应和抗病途径中发挥中心节点调节作用。结论本研究证实，OsSAPK9通过与OsSGT1的互作，在水稻盐胁迫反应和抗病性中发挥正调控作用。
BackgroundSalt stress and bacterial blight caused byXanthomonas oryzaepv.oryzae(Xoo) are key limiting factors of rice (Oryza sativaL.) yields. Members of sucrose non-fermenting 1 (SNF1)-related protein kinase 2 (SnRK2), which is a family of plant-specific Ser/Thr kinases, are important components of signaling pathways involved in plant developmental processes and responses to stresses. There are 10 members of the SnRK2 family in rice; however, their functions are poorly understood, as are the underlying molecular mechanisms.ResultsIn this study, we found thatOsSAPK9,which belongs to the SnRK2 family, positively regulated salt-stress tolerance and strain-specific resistance to bacterial blight in rice. RNA sequencing revealed that there were 404 and 1324 genes differentially expressed inOsSAPK9-RNAiin comparison with wild-type plants under salt-stress conditions and afterXooinoculation, respectively, which participate in basic metabolic processes. In total, 65 common differentially expressed genes involved mainly in defense responses were detected both under salt-stress conditions and afterXooinoculation. Moreover, in vivo and in vitro experiments demonstrated that OsSAPK9 forms a protein complex with the molecular chaperones OsSGT1 and OsHsp90, and transgenic plants overexpressingOsSGT1exhibited decreased tolerances to salt stress and significantly increased resistance levels to bacterial blight. Thus, OsSAPK9 may function as a center node regulator of salt-stress responses and disease-resistance pathways through its interaction with OsSGT1 in rice.ConclusionThis study confirms that OsSAPK9 functions as a positive regulator of salt-stress responses and disease resistance through its interaction with OsSGT1 in rice.
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影响因子：
5.3
作者：
通讯作者：
--
赋予水稻耐旱性的转录因子基因 OsDRAP1 的表征。
影响因子：
5.6
作者：
Huang L;Wang Y;Wang W;Zhao X;Qin Q;Sun F;Hu F;Zhao Y;Li Z;Fu B;Li Z
通讯作者：
Li Z
DOI：
--
发表时间：
1973
期刊：
Plant disease reporter
影响因子：
--
作者：
H. E. Kauffman;A. Reddy;S. Hsieh;S. Merca
通讯作者：
H. E. Kauffman;A. Reddy;S. Hsieh;S. Merca
DOI：
--
发表时间：
1971
期刊：
--
影响因子：
--
作者：
S. Yoshida;D. Forno;J. Cock
通讯作者：
S. Yoshida;D. Forno;J. Cock
影响因子：
4.6
作者：
Romualdi, C;Bortoluzzi, S;Danieli, GA
通讯作者：
Danieli, GA