Characterization of Transcription Factor Gene OsDRAP1 Conferring Drought Tolerance in Rice.
Characterization of Transcription Factor Gene OsDRAP1 Conferring Drought Tolerance in Rice.
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赋予水稻耐旱性的转录因子基因 OsDRAP1 的表征。
DOI：
10.3389/fpls.2018.00094
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发表时间：
2018
影响因子：
5.6
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Huang L;Wang Y;Wang W;Zhao X;Qin Q;Sun F;Hu F;Zhao Y;Li Z;Fu B;Li Z
过量表达和RNA干扰OsDRAP1转基因水稻植株表现出显著的抗旱性提高和降低，但同时也伴随着对生长发育和产量的负面影响。脱水反应元件结合(DREB)基因是重要的转录因子，在植物的非生物胁迫耐受性中起着至关重要的作用。在本研究中，我们对水稻中一个与DREB2类似的基因OsDRAP1进行了功能鉴定。OsDRAP1基因启动子区域含有许多顺式元件，在水稻各器官(主要是维管组织)中均有表达，并受多种环境胁迫和植物激素的诱导。过量表达OsDRAP1基因植株的DT显着提高，而OsDRAP1 RNA干扰植株的DT显着降低，同时也对植株的发育和产量产生了显着的负面影响。过表达OsDRAP1基因对干旱胁迫下转基因水稻植株的水分平衡、氧化还原动态平衡和维管发育具有积极的影响。OsDRAP1与多种基因/蛋白相互作用，并能激活包括OsCBSX3等重要转录因子在内的许多DT下游相关基因以响应干旱胁迫，表明OsDRAP1介导的DT途径涉及复杂的基因网络。这些结果为进一步了解OsDRAP1介导的基因网络及其相关的表型效应奠定了基础。
HIGHLIGHTS Overexpressing and RNA interfering OsDRAP1 transgenic rice plants exhibited significantly improved and reduced drought tolerance, but accompanied with negative effects on development and yield. The dehydration responsive element binding (DREBs) genes are important transcription factors which play a crucial role in plant abiotic stress tolerances. In this study, we functionally characterized a DREB2-like gene, OsDRAP1 conferring drought tolerance (DT) in rice. OsDRAP1, containing many cis-elements in its promoter region, was expressed in all organs (mainly expressed in vascular tissues) of rice, and induced by a variety of environmental stresses and plant hormones. Overexpressing OsDRAP1 transgenic plants exhibited significantly improved DT; while OsDRAP1 RNA interfering plants exhibited significantly reduced DT which also accompanied with significant negative effects on development and yield. Overexpression of OsDRAP1 has a positive impact on maintaining water balance, redox homeostasis and vascular development in transgenic rice plants under drought stress. OsDRAP1 interacted with many genes/proteins and could activate many downstream DT related genes, including important transcription factors such as OsCBSX3 to response drought stress, indicating the OsDRAP1-mediated pathways for DT involve complex genes networks. All these results provide a basis for further complete understanding of the OsDRAP1 mediated gene networks and their related phenotypic effects.
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