A retrospective study of community-acquired Clostridium difficile infection in southwest China.
A retrospective study of community-acquired Clostridium difficile infection in southwest China.
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DOI：
10.1038/s41598-018-21762-7
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发表时间：
2018-03-05
影响因子：
4.6
通讯作者：
Lu J
中科院分区：
文献类型：
--
作者：
Liao F;Li W;Gu W;Zhang W;Liu X;Fu X;Xu W;Wu Y;Lu J
为了解西南地区社区获得性艰难梭菌感染（CA-CDI）的流行情况和特点，我们进行了一项横断面研究。对978例腹泻患者粪便标本进行毒力基因筛查。进行细菌培养，并通过PCR核糖体分型和多位点序列分型对分离株进行鉴定。粪便标本中tcdA和/或tcdB基因检出率为14.11%（138/978）。共55个C。艰难梭菌占5.62%。儿童组与成人组毒素基因阳性率及分离结果差异无统计学意义。但两组在粪便性状、住院前腹泻次数等临床特征上存在差异。儿童多见水样便，成人多见血便;儿童就诊前腹泻次数多≤3次，成人腹泻次数多>3次。CA-CDI的独立危险因素为发热。ST 35/RT 046（18.18%）、ST 54/RT 012（14.55%）、ST 3/RT 001（14.55%）和ST 3/RT 009（12.73%）是分布最广的基因型。ST 35/RT 046、ST 3/RT 001和ST 3/RT 009在儿童患者中较常见，而ST 54/RT 012在成人患者中较常见。云南省CA-CDI的流行率较高，儿童和成人CA-CDI的分离株具有异质性。
To identify the prevalence and characteristics of community-acquired Clostridium difficile infection (CA-CDI) in southwest China, we conducted a cross-sectional study. 978 diarrhea patients were enrolled and stool specimens’ DNA was screened for virulence genes. Bacterial culture was performed and isolates were characterized by PCR ribotyping and multilocus sequence typing. Toxin genes tcdA and/or tcdB were found in 138/978 (14.11%) cases for fecal samples. A total of 55 C. difficile strains were isolated (5.62%). The positive rate of toxin genes and isolation results had no statistical significance between children and adults groups. However, some clinical features, such as fecal property, diarrhea times before hospital treatment shown difference between two groups. The watery stool was more likely found in children, while the blood stool for adults; most of children cases diarrhea ≤3 times before hospital treatment, and adults diarrhea >3 times. Independent risk factor associated with CA-CDI was patients with fever. ST35/RT046 (18.18%), ST54/RT012 (14.55%), ST3/RT001 (14.55%) and ST3/RT009 (12.73%) were the most distributed genotype profiles. ST35/RT046, ST3/RT001 and ST3/RT009 were the commonly found in children patients but ST54/RT012 for adults. The prevalence of CA-CDI in Yunnan province was relatively high, and isolates displayed heterogeneity between children and adults groups.
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影响因子：
9.4
作者：
Alfa, MJ;Kabani, A;Embil, JM
通讯作者：
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影响因子：
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作者：
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通讯作者：
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3.9
作者：
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通讯作者：
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