Robustness of the microtubule network self-organization in epithelia.
Robustness of the microtubule network self-organization in epithelia.
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DOI：
10.7554/elife.59529
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发表时间：
2021-02-01
期刊：
影响因子：
7.7
通讯作者：
Chumakova L
中科院分区：
文献类型：
--
作者：
Płochocka AZ;Ramirez Moreno M;Davie AM;Bulgakova NA;Chumakova L
生物系统的健壮性对于它们的生存至关重要，然而，对于许多系统来说，它的起源是一个悬而未决的问题。在这里，我们分析一个亚细胞水平的系统，微管细胞骨架。微管自组织成一个网络，沿着这个网络，细胞成分被运送到它们的生物相关位置。虽然单个微管的动力学对生物体的环境和遗传很敏感，但整个网络的类似敏感性也会导致病理。我们的大规模随机模拟表明，在单个细胞中，微管网络的自组织在较大的参数范围内是稳健的。我们利用果蝇上皮细胞的遗传操作在体内证实了这一稳健性。最后，我们的最小数学模型表明，稳健性的来源是微管动力学速率中的时间尺度的分离。总之，我们证明了微管网络的组织规模的自组织只取决于细胞的几何形状和微管负端的分布。
Robustness of biological systems is crucial for their survival, however, for many systems its origin is an open question. Here, we analyze one subcellular level system, the microtubule cytoskeleton. Microtubules self-organize into a network, along which cellular components are delivered to their biologically relevant locations. While the dynamics of individual microtubules is sensitive to the organism’s environment and genetics, a similar sensitivity of the overall network would result in pathologies. Our large-scale stochastic simulations show that the self-organization of microtubule networks is robust in a wide parameter range in individual cells. We confirm this robustness in vivo on the tissue-scale using genetic manipulations of Drosophila epithelial cells. Finally, our minimal mathematical model shows that the origin of robustness is the separation of time-scales in microtubule dynamics rates. Altogether, we demonstrate that the tissue-scale self-organization of a microtubule network depends only on cell geometry and the distribution of the microtubule minus-ends.
DOI：
10.4161/bioa.29070
发表时间：
2014-03
期刊：
Bioarchitecture
影响因子：
--
作者：
Dehmelt L
通讯作者：
Dehmelt L
分离的大鼠肝细胞中微管蛋白和微管室的定量分析。
影响因子：
7.8
作者：
Reaven, E P;Cheng, Y;Miller, M D
通讯作者：
Miller, M D