Changes in the protein expression of hedgehog and patched-1 in perisutural tissues induced by cranial distraction.
Changes in the protein expression of hedgehog and patched-1 in perisutural tissues induced by cranial distraction.
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颅骨牵张引起的缝周组织中hedgehog和patched-1蛋白表达的变化。
DOI：
10.1097/00006534-200208000-00024
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发表时间：
2002
影响因子：
3.6
通讯作者：
Mooney,MarkP
中科院分区：
文献类型：
--
作者：
Nott,RhodaL;Stelnicki,EricJ;Mack,JudithA;Ben,Yixin;Mitchell,Ronal;Mooney,MarkP
随着现代微创治疗的发展，牵引的概念被应用于颅缝早闭的治疗。虽然特定的基因突变已被确定在颅缝早闭患者，在基因表达的变化引起的颅骨牵引尚未进行探讨。在兔颅缝早闭模型中评价颅骨牵张对hedgehog和patched-1表达的影响。将家兔（n= 8）分为4组：受累家兔、野生型家兔、颅骨牵张受累家兔和牵张野生型家兔。使用免疫组织化学在四个领域：缝合线，骨内膜，骨膜和骨细胞，印度刺猬，音刺猬，沙漠刺猬和他们的受体，补丁-1的表达检查缝合线周围组织。比较两个实验组：（1）牵引前野生型与牵引后野生型，（2）牵引前骨性结合与牵引后骨性结合。分心刺猬蛋白的存在产生了几个变量和有趣的变化。在野生型兔中，主要效应是印度刺猬水平轻度降低。Sonic和Desert hedgehog和patched-1蛋白水平没有变化。在骨性结合兔，牵引的主要影响是减少印度刺猬，音刺猬，补丁-1蛋白水平。尤其是骨膜和骨内膜。正常和受影响的兔子的颅骨牵引差异改变了刺猬和补丁-1蛋白在颅组织中的表达水平和模式。这些结果表明，牵引和骨反应的分子和遗传参数可能是不同的颅缝早闭个体，这可能会影响这些患者的治疗方案。（Plast。重建110：523，2002）。
: With the modern emphasis on minimally invasive therapies, the concept of distraction is being applied in the treatment of craniosynostosis. Although specific genetic mutations have been identified in craniosynostotic patients, changes in the gene expression induced by cranial distraction have not yet been explored. The effects of cranial distraction on hedgehog and patched-1 expression were evaluated in a rabbit model for craniosynostosis. Rabbits (n= 8) were divided into four groups: affected rabbits, wild-type rabbits, affected rabbits subject to cranial distraction, and wild-type rabbits subject to distraction. Perisutural tissue was examined using immunohistochemistry in four areas: suture, endosteum, periosteum, and osteocytes, for the expression of Indian hedgehog, sonic hedgehog, and desert hedgehog and their receptor, patched-1. Two experimental groups were compared:(1) wild-type before distraction to wild-type after distraction, and (2) synostotic before distraction to synostotic after distraction. Distraction produced several variable and interesting changes in hedgehog protein presence. In wildtype rabbits, the predominant effect was a mild decrease in Indian hedgehog levels. Sonic and desert hedgehog and patched-1 protein levels were unchanged. In synostotic rabbits, the predominant effect of distraction was to decrease Indian hedgehog, sonic hedgehog, and patched-1 protein levels. This was especially true in the periosteum and endosteum. Cranial distraction of normal and affected rabbits differentially changed both the expression levels and patterns of the hedgehog and patched-1 proteins in the cranial tissues examined. These results suggest that molecular and genetic parameters of distraction and bone response may be different in craniosynostotic individuals, which may influence treatment protocols in these patients.(Plast. Reconstr. Surg. 110: 523, 2002.)
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影响因子：
3.3
作者：
Mikuni-Takagaki, Y
通讯作者：
Mikuni-Takagaki, Y
影响因子：
4.6
作者：
Hyun-Jung Kim;D. Rice;P. Kettunen;I. Thesleff
通讯作者：
Hyun-Jung Kim;D. Rice;P. Kettunen;I. Thesleff
影响因子：
3.6
作者：
SNYDER, CC;LEVINE, GA;BROWNE, EZ
通讯作者：
BROWNE, EZ
迟发性冠状缝骨融合兔的颅内骨牵引
影响因子：
3.6
作者：
W. Losken;M. Mooney;J. Zoldos;A. Tschakaloff;A. Burrows;T. Smith;G. Cooper;R. Kapucu;M. Siegel
通讯作者：
M. Siegel
影响因子：
0.9
作者：
Toth, BA;Kim, JW;Cedars, M
通讯作者：
Cedars, M