RNA polyadenylation sites on the genomes of microorganisms, animals, and plants.
RNA polyadenylation sites on the genomes of microorganisms, animals, and plants.
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DOI：
10.1371/journal.pone.0079511
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Du D
中科院分区：
文献类型：
--
作者：
Li XQ;Du D
前信使RNA(MRNA)3‘端裂解和随后的多聚腺苷化强烈地调节基因的表达。与上游或下游基序相比，人们对主要王国之间多聚腺苷酸化[PolyA]位点的特征差异知之甚少。我们怀疑精确的聚(A)位点具有非常高的选择性，因此我们使用国家生物技术信息中心(NCBI)核苷酸数据库中的mRNA序列将mRNA聚(A)位点定位在完整和接近完整的基因组上。在这篇文章中，我们描述了在29个最常见的物种(2个真菌，2个原生动物，18个动物和7个植物)中，直接与Poly(A)尾巴连接的mRNA核苷酸[即Poly(A)尾部附着位置]和对应于Poly(A)尾部的第一个腺苷的前mRNA核苷酸[即Poly(A)尾部起始位置]。覆盖这两个位置的最具代表性的前mRNA二核苷酸分别是UA、CA和GA，分别在17、10和2个物种中。多聚(A)尾部起始位置的前mRNA核苷酸通常是腺苷[即A型多聚(A)位点]，有时是尿苷，有时是胞苷或鸟苷。在附件位置的顺序是U>；C&>G，但在起始位置的顺序是A>；>；U>；C≥G。然而，与mRNA的核苷酸组成(碱基组成)相比，在植物中，Poly(A)的尾部连接位置选择了C而不是U，在动物中，无论是在A型还是在非A型PolyA位点上，C和G都选择了U。动物、双子叶植物和单子叶植物在非A-型聚(A)位点的聚(A)尾部附着位置的C/G比值有明显差异。这项对PolyA位点进化的研究表明，PolyA位点内的这两个位置具有不同的核苷酸组成，并且在不同的王国之间是不同的。
Pre–messenger RNA (mRNA) 3′-end cleavage and subsequent polyadenylation strongly regulate gene expression. In comparison with the upstream or downstream motifs, relatively little is known about the feature differences of polyadenylation [poly(A)] sites among major kingdoms. We suspect that the precise poly(A) sites are very selective, and we therefore mapped mRNA poly(A) sites on complete and nearly complete genomes using mRNA sequences available in the National Center for Biotechnology Information (NCBI) Nucleotide database. In this paper, we describe the mRNA nucleotide [i.e., the poly(A) tail attachment position] that is directly in attachment with the poly(A) tail and the pre-mRNA nucleotide [i.e., the poly(A) tail starting position] that corresponds to the first adenosine of the poly(A) tail in the 29 most-mapped species (2 fungi, 2 protists, 18 animals, and 7 plants). The most representative pre-mRNA dinucleotides covering these two positions were UA, CA, and GA in 17, 10, and 2 of the species, respectively. The pre-mRNA nucleotide at the poly(A) tail starting position was typically an adenosine [i.e., A-type poly(A) sites], sometimes a uridine, and occasionally a cytidine or guanosine. The order was U>C>G at the attachment position but A>>U>C≥G at the starting position. However, in comparison with the mRNA nucleotide composition (base composition), the poly(A) tail attachment position selected C over U in plants and both C and G over U in animals, in both A-type and non-A-type poly(A) sites. Animals, dicot plants, and monocot plants had clear differences in C/G ratios at the poly(A) tail attachment position of the non-A-type poly(A) sites. This study of poly(A) site evolution indicated that the two positions within poly(A) sites had distinct nucleotide compositions and were different among kingdoms.
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影响因子：
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作者：
Fuentes, Vanessa;Barrera, Guadalupe;Lopez-Villasenor, Imelda
通讯作者：
Lopez-Villasenor, Imelda
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