PxdA interacts with the DipA phosphatase to regulate peroxisome hitchhiking on early endosomes.
PxdA interacts with the DipA phosphatase to regulate peroxisome hitchhiking on early endosomes.
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DOI：
10.1091/mbc.e20-08-0559
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发表时间：
2021-03-15
影响因子：
3.3
通讯作者：
Reck-Peterson SL
中科院分区：
文献类型：
--
作者：
Salogiannis J;Christensen JR;Songster LD;Aguilar-Maldonado A;Shukla N;Reck-Peterson SL
在典型的基于微管的运输中，衔接蛋白将货物连接到动力蛋白和驱动蛋白马达。最近，一种被称为“搭便车”的替代运输模式被发现，其中货物通过搭已经运动的货物的便车而不是附着在运动蛋白上来实现运动。搭便车在两种丝状真菌中研究得最好，构巢曲霉和玉米黑粉菌。在us玉米、核糖核蛋白复合物、过氧化物酶体、脂滴（LD）和内质网搭便车在早期内涵体（EE）上。以.过氧化物酶体分布突变体A（Peroxisome Distribution Mutant A，PxdA）是一种与EE相关的分子连接体。然而，其他细胞器是否使用PxdA搭便车在EE上尚不清楚，调节搭便车的分子机制也不清楚。在这里，我们发现LD，线粒体和preautophagosomes的适当分布不需要PxdA，这表明PxdA是过氧化物酶体特异性的分子接头。我们确定了两个新的pxdA等位基因，包括一个点突变（R2044P），破坏PxdA的能力，与EE和减少过氧化物酶体运动。我们还确定了一种新的调节过氧化物酶体搭便车，磷酸酶DipA。DipA与EE共定位，其与EE的缔合依赖于PxdA。总之，我们的数据表明，PxdA和DipA磷酸酶是过氧化物酶体搭便车对EE的特定调节剂。
In canonical microtubule-based transport, adaptor proteins link cargoes to dynein and kinesin motors. Recently, an alternative mode of transport known as “hitchhiking” was discovered, where cargoes achieve motility by hitching a ride on already-motile cargoes, rather than attaching to a motor protein. Hitchhiking has been best studied in two filamentous fungi, Aspergillus nidulans and Ustilago maydis. In U. maydis, ribonucleoprotein complexes, peroxisomes, lipid droplets (LDs), and endoplasmic reticulum hitchhike on early endosomes (EEs). In A. nidulans, peroxisomes hitchhike using a putative molecular linker, peroxisome distribution mutant A (PxdA), which associates with EEs. However, whether other organelles use PxdA to hitchhike on EEs is unclear, as are the molecular mechanisms that regulate hitchhiking. Here we find that the proper distribution of LDs, mitochondria, and preautophagosomes do not require PxdA, suggesting that PxdA is a peroxisome-specific molecular linker. We identify two new pxdA alleles, including a point mutation (R2044P) that disrupts PxdA’s ability to associate with EEs and reduces peroxisome movement. We also identify a novel regulator of peroxisome hitchhiking, the phosphatase DipA. DipA colocalizes with EEs and its association with EEs relies on PxdA. Together, our data suggest that PxdA and the DipA phosphatase are specific regulators of peroxisome hitchhiking on EEs.
影响因子：
3.7
作者：
Suresh S;Abdurehman L;Osmani AH;Osmani SA
通讯作者：
Osmani SA