Human enteroendocrine cell responses to infection with Chlamydia trachomatis: a microarray study.
Human enteroendocrine cell responses to infection with Chlamydia trachomatis: a microarray study.
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DOI：
10.1186/1757-4749-6-24
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发表时间：
2014
期刊：
影响因子：
4.2
通讯作者：
Lindberg G
中科院分区：
文献类型：
--
作者：
Dlugosz A;Muschiol S;Zakikhany K;Assadi G;D'Amato M;Lindberg G
肠内分泌细胞（EEC）是高度特化的细胞，产生对肠道正常功能至关重要的信号分子。最近，我们发现改变蛋白质分布在衣原体感染的EEC在体外。本研究的目的是进行微阵列分析的响应模式，从大肠和小肠感染体外，沙眼衣原体感染作为一个模型。两条人体EEC线：用培养的C.沙眼血清型LGV II菌株434（ATCC VR-902 B）。青霉素G用于诱导持续感染。我们使用微阵列分析（AffyssineGeneChip®）来研究感染的不同阶段基因表达的变化。在主动和持续感染后24小时，LCC-18中的66和411个基因以及CNDT-2细胞中的68和170个基因分别显示与未感染细胞相比> 2倍的平均表达比率。这些基因编码调控细胞凋亡、细胞分化、转录调节、细胞因子活性、胺生物合成和囊泡转运的因子。我们发现持续感染和非感染细胞之间的基因转录水平的显着差异，在10个基因编码不同的溶质载体转运蛋白（SLC）和5个基因相关的内分泌功能（GABARAPL1，GRIP1，DRD2，SYT5和SYT7）。受感染的EEC细胞表现出与囊泡运输、分泌和神经递质相关的细胞类型特异性模式。EEC在调节肠道运动中起着关键作用，肠内分泌功能受损可导致运动障碍。
Enteroendocrine cells (EEC) are highly specialized cells producing signalling molecules vital to the normal functions of the gut. Recently, we showed altered protein distribution in Chlamydia infected EEC in vitro. The aim of this study was to perform a microarray analysis of the response pattern of EEC from both large and small bowel to infection in vitro, using Chlamydia trachomatis infection as a model. Two human EEC lines: LCC-18, derived from a neuroendocrine colonic tumour, and CNDT-2, derived from a small intestinal carcinoid, were infected using cultured C. trachomatis serovar LGV II strain 434 (ATCC VR-902B). Penicillin G was used to induce persistent infection. We used microarray analysis (Affymetrix GeneChip®) for studying changes in gene expression at different stages of infection. Twenty-four hours after active and persistent infection, 66 and 411 genes in LCC-18 and 68 and 170 genes in CNDT-2 cells, respectively showed mean expression ratios >2-fold compared to non-infected cells. These genes encoded factors regulating apoptosis, cell differentiation, transcription regulation, cytokine activity, amine biosynthesis and vesicular transport. We found significant differences in gene transcription levels between persistently infected and non-infected cells in 10 genes coding for different solute carrier transporters (SLC) and in 5 genes related to endocrine function (GABARAPL1, GRIP1, DRD2, SYT5 and SYT7). Infected EEC cells exhibit cell-type specific patterns related to vesicular transport, secretion and neurotransmitters. EEC play a pivotal role in regulation of gut motility and an impairment of enteroendocrine function can contribute to motility disorders.
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DOI：
10.1073/pnas.011404098
发表时间：
2001-01-02
影响因子：
11.1
作者：
Li, C;Wong, WH
通讯作者：
Wong, WH
影响因子：
3.1
作者：
Eickhoff, Meike;Thalmann, Jessica;Klos, Andreas
通讯作者：
Klos, Andreas
影响因子：
4
作者：
Harrison, Elizabeth;Lal, Simon;McLaughlin, John T.
通讯作者：
McLaughlin, John T.
DOI：
10.1152/ajpgi.00552.2005
发表时间：
2006-11-01
影响因子：
4.5
作者：
Kidd, M.;Modlin, I. M.;Champaneria, M. C.
通讯作者：
Champaneria, M. C.
影响因子：
4
作者：
Kirchgessner, Annette L.
通讯作者：
Kirchgessner, Annette L.