Light and the circadian clock mediate time-specific changes in sensitivity to UV-B stress under light/dark cycles.
Light and the circadian clock mediate time-specific changes in sensitivity to UV-B stress under light/dark cycles.
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DOI：
10.1093/jxb/eru339
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发表时间：
2014-11
影响因子：
6.9
通讯作者：
Farré EM
中科院分区：
文献类型：
--
作者：
Takeuchi T;Newton L;Burkhardt A;Mason S;Farré EM
生理时钟和光影响UV-B应激反应的时间依赖性。在拟南芥中，生物钟成分以基因特异性的方式调节uv -b介导的表达。在拟南芥中，生物钟调节uv - b介导的基因表达变化。研究表明，生物钟成分能够以一种基因特异性的方式抑制UV- b诱导的基因表达，并作用于COP1 （CONSTITUTIVE PHOTOMORPHOGENIC 1）和UVR8 （UV抗性位点8）对UV- b的初始感知的下游。例如，UV-B诱导的ELIP1 （EARLY LIGHT INDUCIBLE PROTEIN 1）和PRR9 （PSEUDO-RESPONSE REGULATOR 9）由LUX （LUX ARRYTHMO）、ELF4 （EARLY开花4）和ELF3直接调控。此外，还观察到植物对UV-B损伤的敏感性随时间的变化。除生物钟突变体外，野生型拟南芥在夜间对UV-B处理的敏感性高于光照期。在6小时光照和6小时黑暗的短周期下进行的实验表明，野生型幼苗在黑暗中对UV-B的胁迫敏感性只在主观的夜间增加，而在主观的白天没有增加。相比之下，生物钟受损的拟南芥突变体的胁迫敏感性仍然受到主观白天光照条件的影响。综上所述，结果表明，时钟和光在一天中的不同时间调节植物对UV-B胁迫的敏感性。
The circadian clock and light influence the time dependence of the UV-B stress response. Circadian clock components regulate UV-B-mediated expression in a gene-by-gene-specific manner in Arabidopsis. In Arabidopsis, the circadian clock regulates UV-B-mediated changes in gene expression. Here it is shown that circadian clock components are able to inhibit UV-B-induced gene expression in a gene-by-gene-specific manner and act downstream of the initial UV-B sensing by COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) and UVR8 (UV RESISTANCE LOCUS 8). For example, the UV-B induction of ELIP1 (EARLY LIGHT INDUCIBLE PROTEIN 1) and PRR9 (PSEUDO-RESPONSE REGULATOR 9) is directly regulated by LUX (LUX ARRYTHMO), ELF4 (EARLY FLOWERING 4), and ELF3. Moreover, time-dependent changes in plant sensitivity to UV-B damage were observed. Wild-type Arabidopsis plants, but not circadian clock mutants, were more sensitive to UV-B treatment during the night periods than during the light periods under diel cycles. Experiments performed under short cycles of 6h light and 6h darkness showed that the increased stress sensitivity of plants to UV-B in the dark only occurred during the subjective night and not during the subjective day in wild-type seedlings. In contrast, the stress sensitivity of Arabidopsis mutants with a compromised circadian clock was still influenced by the light condition during the subjective day. Taken together, the results show that the clock and light modulate plant sensitivity to UV-B stress at different times of the day.
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