Lipidomics dataset of PTEN deletion-induced optic nerve regeneration mouse model.
Lipidomics dataset of PTEN deletion-induced optic nerve regeneration mouse model.
复制标题
PTEN缺失诱导视神经再生小鼠模型的脂质组学数据集。
DOI：
10.1016/j.dib.2020.106699
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发表时间：
2021-03
期刊：
影响因子：
1.2
通讯作者：
Bhattacharya SK
中科院分区：
文献类型：
--
作者：
Arcuri J;Hegarty S;He Z;Bhattacharya SK
视神经是成年哺乳动物中枢神经系统（CNS）的一部分，损伤后再生能力有限。磷酸酶和张力蛋白同源物（PTEN）的缺失，一种PI 3激酶/Akt通路的负调节剂，已被证明促进视神经损伤后视网膜神经节细胞（RGC）的再生。我们提出了成年PTENloxP/loxP小鼠的脂质体，其玻璃体内注射表达Cre的腺相关病毒（AAV-Cre）作为CNS神经再生的模型。在4周龄时，向PTENloxP/loxP小鼠玻璃体内注射2-3 μ 1 AAV-Cre（KO）或AAV-PLAP（对照），两周后进行视神经挤压。在压碎后的指定时间点（0天、7天、14天），将小鼠安乐死，并立即解剖出视神经，然后在干冰上快速冷冻。使用改良的Bligh和Dyer方法从视神经中提取脂质，然后使用Q-Exactive Orbitrap仪器与Difa 600 HPLC联用进行液相色谱-质谱（LC MS-MS）脂质分析。使用LipidSearch 4.2分析原始扫描，并通过Metaboanalyst 4.0进行统计分析。该数据可在代谢组学研究中心获得，研究ID ST 001477。
The optic nerve is part of the mammalian adult central nervous system (CNS) and has limited capability to regenerate after injury. Deletion of phosphatase and tensin homolog (PTEN), a negative regulator of the PI3 kinase/Akt pathway, has been shown to promote regeneration in retinal ganglion cells (RGCs) after optic nerve injury. We present the lipidome of adult PTENloxP/loxP mice subjected to intravitreal injection of adeno-associated viruses expressing Cre (AAV-Cre) as a model of CNS neuroregeneration. At 4 weeks old, PTENloxP/loxP mice were intravitreally-injected with 2–3 μl of either AAV-Cre (KO) or AAV-PLAP (control), and two weeks later optic nerve crush was performed. At indicated time-points after crush (0 days, 7 days, 14 days), mice were euthanized and optic nerves were immediately dissected out, and then flash frozen on dry ice. A modified Bligh and Dyer method was used for lipid extraction from the optic nerves, followed by liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Accela 600 HPLC. The raw scans were analysed with LipidSearch 4.2 and the statistical analysis was conducted through Metaboanalyst 4.0. This data is available at Metabolomics Workbench, study ID ST001477.
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影响因子：
1.2
作者：
Trzeciecka, Anna;Carmy, Tal;Bhattacharya, Sanjoy K.
通讯作者：
Bhattacharya, Sanjoy K.
DOI：
10.1126/science.1161566
发表时间：
2008-11-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park KK;Liu K;Hu Y;Smith PD;Wang C;Cai B;Xu B;Connolly L;Kramvis I;Sahin M;He Z
通讯作者：
He Z
影响因子：
1.2
作者：
Arcuri, Jennifer;Liu, Yuan;Bhattacharya, Sanjoy K.
通讯作者：
Bhattacharya, Sanjoy K.
影响因子：
1.2
作者：
Trzeciecka, Anna;Stark, David T.;Caprioli, Joseph
通讯作者：
Caprioli, Joseph