Measuring immunity to SARS-CoV-2 infection: comparing assays and animal models.
Measuring immunity to SARS-CoV-2 infection: comparing assays and animal models.
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DOI：
10.1038/s41577-020-00471-1
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Davenport MP
中科院分区：
文献类型：
--
作者：
Khoury DS;Wheatley AK;Ramuta MD;Reynaldi A;Cromer D;Subbarao K;O'Connor DH;Kent SJ;Davenport MP
2019冠状病毒病（COVID - 19）研究的快速扩大催生了大量潜在的疫苗和免疫疗法，同时也产生了数量相当多的体外检测和体内模型来衡量其有效性。这些检测大体上有相同的最终目标——预测预防性和治疗性干预措施在人体中的临床疗效。然而，不同干预措施的明显效力在不同检测和动物模型之间可能有很大差异，从而导致对临床疗效的预测大不相同。以目前的研究速度，完全统一实验方法可能是难以实现的。然而，在此我们分析了一些现有的用于测量抗体介导的病毒中和作用的检测方法以及严重急性呼吸综合征冠状病毒2（SARS - CoV - 2）感染的动物模型，并提供了一个框架用于比较不同研究之间的结果以及调和在干预效果方面观察到的差异。最后，我们提出如何优化这些检测方法，以便更好地比较体外和动物研究的结果，从而加速进展。
你是刚开始进行病毒研究并试图解读关于严重急性呼吸综合征冠状病毒2（SARS - CoV - 2）免疫的不断增多的文献吗？在这里，作者比较了用于测量对SARS - CoV - 2感染的免疫力的不同检测方法和动物模型，并调和了在不同检测中评估的抗体明显效力的差异。
The rapid scale-up of research on coronavirus disease 2019 (COVID-19) has spawned a large number of potential vaccines and immunotherapies, accompanied by a commensurately large number of in vitro assays and in vivo models to measure their effectiveness. These assays broadly have the same end-goal — to predict the clinical efficacy of prophylactic and therapeutic interventions in humans. However, the apparent potency of different interventions can vary considerably between assays and animal models, leading to very different predictions of clinical efficacy. Complete harmonization of experimental methods may be intractable at the current pace of research. However, here we analyse a selection of existing assays for measuring antibody-mediated virus neutralization and animal models of infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and provide a framework for comparing results between studies and reconciling observed differences in the effects of interventions. Finally, we propose how we might optimize these assays for better comparison of results from in vitro and animal studies to accelerate progress. Are you new to virus research and trying to interpret the ever-expanding literature on immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)? Here, the authors compare the different assays and animal models used to measure immunity to SARS-CoV-2 infection and reconcile differences in apparent potency of antibodies assessed in different assays.
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