Productive Infection of Human Breast Cancer Cell Lines with Human Cytomegalovirus (HCMV).
Productive Infection of Human Breast Cancer Cell Lines with Human Cytomegalovirus (HCMV).
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DOI：
10.3390/pathogens10060641
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发表时间：
2021-05-23
期刊：
影响因子：
--
通讯作者：
Spencer JV
中科院分区：
文献类型：
--
作者：
Branch KM;Garcia EC;Chen YM;McGregor M;Min M;Prosser R;Whitney N;Spencer JV
Breast cancer is the leading cause of cancer deaths among women worldwide. There are many known risk factors for breast cancer, but the role of infectious disease remains unclear. Human cytomegalovirus (HCMV) is a widespread herpesvirus that usually causes little disease. Because HCMV has been detected in breast tumor biopsy samples and is frequently transmitted via human breast milk, we investigated HCMV replication in breast tumor cells. Four human breast cancer cell lines with different expression profiles for the key diagnostic markers of the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2), were infected with a bacterial artificial chromosome-derived HCMV clinical strain TB40/E tagged with green fluorescent protein (GFP). Fluorescence microscopy confirmed that all four breast cancer cell lines supported virus entry. RNA was isolated from infected cells and the expression of immediate early (UL123), early (UL54), and late (UL111A) genes was confirmed using PCR. Viral proteins were detected by immunoblotting, and viral progeny were produced during the infection of breast tumor cells, as evidenced by subsequent infection of fibroblasts with culture supernatants. These results demonstrate that breast tumor cells support productive HCMV infection and could indicate that HCMV replication may play a role in breast cancer progression.
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影响因子：
4.7
作者：
Herbein G;Kumar A
通讯作者：
Kumar A
影响因子：
4.7
作者：
Richardson, A
通讯作者：
Richardson, A
影响因子：
3.7
作者：
Alibek K;Kakpenova A;Mussabekova A;Sypabekova M;Karatayeva N
通讯作者：
Karatayeva N
影响因子：
4.7
作者：
Mohamed HT;El-Shinawi M;Nouh MA;Bashtar AR;Elsayed ET;Schneider RJ;Mohamed MM
通讯作者：
Mohamed MM
DOI：
10.1073/pnas.1710799114
发表时间：
2017-09-26
影响因子：
11.1
作者：
Oberstein, Adam;Shenk, Thomas
通讯作者：
Shenk, Thomas