Mucocutaneous Junction of Eyelid and Lip: A Study of the Transition Zone Using Epithelial Cell Markers
Mucocutaneous Junction of Eyelid and Lip: A Study of the Transition Zone Using Epithelial Cell Markers
复制标题
眼睑和嘴唇的皮肤粘膜交界处：使用上皮细胞标记物的过渡区研究
作者：
A. Riau;V. Barathi;R. Beuerman
目的：上皮细胞标志物在小鼠眼睑和嘴唇的表达进行了研究，以了解粘膜和皮肤上皮细胞的相互作用的性质，它们如何形成粘膜皮肤交界处（MCJ）。研究方法：用细胞角蛋白（CKs）：CK 1、CK 4、CK 5、CK 6、CK 10、CK 13、CK 14和CK 19、聚丝蛋白、外皮蛋白和连接蛋白43对正常小鼠眼睑和唇组织的冷冻切片进行免疫化学检查。结果如下：MCJ的表达模式各不相同，粘膜上皮中没有CK 1、CK 10和聚丝蛋白;皮肤表皮中没有CK 4、CK 6和CK 13。CK 5和CK 14在全层皮肤、MCJ和粘膜中一致表达。CK 19表达基底，而外皮蛋白阳性细胞被发现在皮肤，MCJ和粘膜表面。连接蛋白43存在于MCJ、皮肤和唇粘膜中;然而，在睑结膜中几乎没有表达。结论：MCJ可能是粘膜上皮细胞分化活动的集中点。眼睑和嘴唇中染色模式的相似性表明，内部和外部环境之间共享相互作用的这些位点的形成采用了相似的细胞机制。
Purpose: The expression of epithelial cell markers in the mouse eyelid and lip was investigated in order to understand the nature of the interactions of mucosal and skin epithelium as to how they form the mucocutaneous junction (MCJ). Methods: Cryosections of eyelid and lip tissue from normal mice were examined immunohistochemically with cytokeratins (CKs): CK1, CK4, CK5, CK6, CK10, CK13, CK14, and CK19; filaggrin; involucrin; and connexin 43. Results: The expression pattern varied across the MCJ, with the absence of CK1, CK10, and filaggrin in the mucosal epithelium; and CK4, CK6, and CK13 in the skin epidermis. CK5 and CK14 were consistently expressed in full-thickness skin, MCJ, and mucosa. CK19 was expressed basally, while involucrin-positive cells were found superficially in skin, MCJ, and mucosa. Connexin 43 was present in the MCJ, skin, and labial mucosa; however, little to no expression was seen in the palpebral conjunctiva. Conclusion: The MCJ may be a focal point of mucosal epithelial cell differentiation activities. The similarity of staining patterns in the eyelid and lip suggests that the formation of these sites of shared interaction between the internal and external environment employs similar cellular mechanisms.
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小鼠皮肤在毛发周期的早期毛发生长初期特别容易受到肿瘤的影响：可能涉及毛囊干细胞。
DOI：
10.1111/1523-1747.ep12366045
发表时间：
1993
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Miller,SJ;Wei,ZG;Wilson,C;Dzubow,L;Sun,TT;Lavker,RM
通讯作者：
Lavker,RM
影响因子：
4.4
作者：
Z. Wei;R. Wu;R. Lavker;T. Sun
通讯作者：
Z. Wei;R. Wu;R. Lavker;T. Sun
影响因子：
4.4
作者：
Zhiqian Wei;G. Cotsarelis;T. Sun;R. M. Lavker
通讯作者：
Zhiqian Wei;G. Cotsarelis;T. Sun;R. M. Lavker
影响因子：
56.9
作者：
LAVKER, RM;SUN, TT
通讯作者：
SUN, TT