Sebocytes contribute to melasma onset.
Sebocytes contribute to melasma onset.
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皮脂腺细胞有助于黄褐斑的发生。
DOI：
10.1016/j.isci.2022.103871
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发表时间：
2022-03-18
期刊：
影响因子：
5.8
通讯作者：
Picardo M
中科院分区：
文献类型：
--
作者：
Flori E;Mastrofrancesco A;Mosca S;Ottaviani M;Briganti S;Cardinali G;Filoni A;Cameli N;Zaccarini M;Zouboulis CC;Picardo M
黄褐斑是一种具有光老化特征的色素沉着障碍，表现在特定的面部区域，富含皮脂腺(SGS)。为了探讨SGS在SZ95 SG细胞发病中的可能作用，研究了SZ95 SG细胞株在单次和重复紫外线(UVA)照射下促黑素生成因子和炎症因子的表达。UVA上调α-MSH、EDN1、b-FGFs、SCF、炎性细胞因子和介质的持续产生。照射后的SZ95皮脂细胞条件培养液增加了黑素细胞的色素沉着，增加了成纤维细胞中调节黑素生成的衰老标志物、促炎细胞因子和生长因子的表达。皮肤外植体的共培养实验证实了皮脂细胞在促进黑素生成方面的作用。对黄褐斑患者皮脂的分析表明，在体内，皮损区域的皮脂细胞表达体外观察到的UVA激活的通路标志。我们的结果表明，皮脂细胞是黄褐斑发病的因素之一，诱导皮肤细胞的长期刺激，导致局部皮肤老化和色素沉着。受辐射的皮脂细胞产生并上调因子α-MSH、EDN1、SCF。和b-FGF皮脂细胞衍生因子驱动成纤维细胞和黑素细胞行为的改变皮脂细胞参与皮肤细胞串扰调节色素沉着皮脂细胞局部支持黄褐斑的炎症和光老化环境生物科学；生物化学；分子生物学
Melasma is a hyperpigmentary disorder with photoaging features, whose manifestations appear on specific face areas, rich in sebaceous glands (SGs). To explore the SGs possible contribution to the onset, the expression of pro-melanogenic and inflammatory factors from the SZ95 SG cell line exposed to single or repetitive ultraviolet (UVA) radiation was evaluated. UVA up-modulated the long-lasting production of α-MSH, EDN1, b-FGF, SCF, inflammatory cytokines and mediators. Irradiated SZ95 sebocyte conditioned media increased pigmentation in melanocytes and the expression of senescence markers, pro-inflammatory cytokines, and growth factors regulating melanogenesis in fibroblasts cultures. Cocultures experiments with skin explants confirmed the role of sebocytes on melanogenesis promotion. The analysis on sebum collected from melasma patients demonstrated that in vivo sebocytes from lesional areas express the UVA-activated pathways markers observed in vitro. Our results indicate sebocytes as one of the actors in melasma pathogenesis, inducing prolonged skin cell stimulation, contributing to localized dermal aging and hyperpigmentation. Irradiated sebocytes produce and upregulate the factors α-MSH, EDN1, SCF. and b-FGF Sebocyte-derived factors drive modifications of fibroblasts and melanocyte behavior Sebocytes participate in the skin cells cross-talk regulating pigmentation Sebocytes locally support the inflammatory and photo-aged environment in melasma Biological sciences; Biochemistry; Molecular biology
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