Characterization of a mesenchymal stem cell line that differentiates to bone and provides niches supporting mouse and human hematopoietic stem cells
Characterization of a mesenchymal stem cell line that differentiates to bone and provides niches supporting mouse and human hematopoietic stem cells
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分化为骨并提供支持小鼠和人类造血干细胞的生态位的间充质干细胞系的表征
DOI：
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
J. Sharp
中科院分区：
文献类型：
--
作者：
Sonal R. Tuljapurkar;J. Jackson;Susan K. Brusnahan;Barbara J. O’Kane;J. Sharp
需要鉴定具有原代多能间充质基质细胞（MSC）特性的小鼠细胞系，以便于使用小鼠模型评估骨形成、造血和再生医学细胞疗法的机制。原代鼠MSC在品系之间变化，难以在体外生长并且具有不一致的性质。本研究的主要目的是建立OMA-AD细胞作为一个合适的模型系统，进行MSC，骨形成和造血的研究。通过C57 BL/6 J小鼠骨髓（BM）细胞的差异胰蛋白酶消化分离OMA-AD细胞。然后将细胞再传代、克隆和表征。OMA-AD细胞具有永生性和非致瘤性，易于分化为包括骨在内的所有间充质细胞类型，支持小鼠和人造血，并且具有免疫抑制性。我们的结果表明，OMA-AD细胞具有原代MSC的特性。此外，这些细胞生长迅速，一致，从而促进未来的骨形成，造血和再生医学间充质细胞的研究。
Identification of mouse cell lines with properties of primary multipotential mesenchymal stromal cells (MSC) is required to facilitate the use of mouse models for evaluation of mechanisms in bone formation, hematopoiesis and cellular therapies for regenerative medicine. Primary murine MSC vary between strains, are difficult to grow in vitro and have inconsistent properties. The main aim of the study was to establish OMA-AD cells as an appropriate model system to conduct studies on MSC, bone formation and hematopoiesis. OMA-AD cells were isolated by differential trypsinization of C57BL/6J mouse bone marrow (BM) cells. The cells were then repassaged, cloned and characterized. OMA-AD cells were immortal and non-tumorigenic, differentiated readily to all mesenchymal cell types including bone, supported mouse and human hematopoiesis and were immunosuppressive. Our results demonstrated that OMA-AD cells possessed the properties of primary MSC. In addition, these cells grew readily and consistently, thereby facilitating future studies of bone formation, hematopoiesis and mesenchymal cells for regenerative medicine.
DOI：
10.1073/pnas.92.11.4857
发表时间：
1995-05-23
影响因子：
11.1
作者：
PEREIRA, RF;HALFORD, KW;PROCKOP, DJ
通讯作者：
PROCKOP, DJ
c-Kit 阴性造血干细胞体外生长条件的鉴定。
影响因子：
20.3
作者：
Klarmann,Kim;Ortiz,Mariaestela;Davies,Meghan;Keller,JonathanR
通讯作者：
Keller,JonathanR
DOI：
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发表时间：
1984
期刊：
Kroc Foundation series
影响因子：
--
作者：
Crouse,DA;Mann,SL;Sharp,JG
通讯作者：
Sharp,JG