Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis and Necroptosis via Inhibiting Endoplasmic Reticulum Stress.
Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis and Necroptosis via Inhibiting Endoplasmic Reticulum Stress.
复制标题
牛磺熊去氧胆酸通过抑制内质网应激保护髓核细胞免受压迫诱导的细胞凋亡和坏死性凋亡
DOI：
10.1155/2018/6719460
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Shao Z
中科院分区：
文献类型：
--
作者：
Wang W;Qing X;Wang B;Ma K;Wei Y;Shao Z
Tauroursodeoxycholic acid (TUDCA) is a kind of hydrophilic bile acid, which could protect cells from death via inhibiting endoplasmic reticulum (ER) stress. However, the role of TUDCA in compression-induced intervertebral disc degeneration (IVDD) has not been elucidated. Here, we used a previously described device to mimic in vivo compression conditions. NP cells treated with DMSO or TUDCA were exposed to compression. Then, cell viability, morphology, and apoptosis were detected. Furthermore, apoptosis-related proteins and necroptosis markers were detected too. To investigate the specific cytoprotective mechanisms of TUDCA in IVDD, we detected the ER morphology by electron microscopy. In addition, the ER stress of nucleus pulposus (NP) cells was quantitatively evaluated by analyzing the level of ER-stress-associated proteins. Our results revealed that TUDCA could protect NP cells from excessive compression-induced death by reducing the apoptosis and necroptosis. In addition, ER stress is involved in pathogenesis of IVDD induced by excessive compression and plays a detrimental role. TUDCA exerts its protective functions by inhibiting ER stress. In conclusion, TUDCA could protect NP cells from compression-induced death, which suggested that treatment by TUDCA may be a potential method to retard IVDD.
登录
查看更多内容
影响因子：
7.2
作者：
Ding, Fan;Shao, Zeng-Wu;Xiong, Li-Ming
通讯作者：
Xiong, Li-Ming
影响因子：
3
作者：
Kluba, T;Niemeyer, T;Gründer, T
通讯作者：
Gründer, T
影响因子：
4
作者：
Paschen, W;Mengesdorf, T
通讯作者：
Mengesdorf, T
影响因子：
3
作者：
BUCKWALTER, JA
通讯作者：
BUCKWALTER, JA
DOI：
10.1152/ajpgi.00483.2010
发表时间：
2011-11-01
影响因子：
4.5
作者：
Seyhun, Ersin;Malo, Antje;Kubisch, Constanze H.
通讯作者：
Kubisch, Constanze H.