Little effect of the tan locus on pigmentation in female hybrids between Drosophila santomea and D. melanogaster.

Little effect of the tan locus on pigmentation in female hybrids between Drosophila santomea and D. melanogaster.
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棕褐色基因座对果蝇圣托米亚和D. melanogaster之间的女性杂种的色素沉着几乎没有影响。

DOI:
10.1016/j.cell.2009.10.033
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发表时间:
2009-12-11
期刊:
影响因子:
64.5
通讯作者:
Coyne JA
Coyne JA
中科院分区:
生物学1区
文献类型:
--
作者:
Matute DR;Butler IA;Coyne JA

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先前对果蝇santomea的研究表明,与其他深色色素的物种相比,其缺乏腹部色素沉着是基于tan顺式调节区的突变,使该基因在果蝇腹部的表达失活。桑托米亚的雄性和雌性我们发现D. santomea雄性与D.雌性黑腹果蝇使我们能够利用后一种物种的遗传工具来研究这种种间色素差异的遗传基础。杂交试验采用D.包括棕褐色在内的黑腹缺陷没有证据表明该位点与D. santomea女性;相反,色素沉着的差异似乎涉及该地区至少四个其他位点。早期的结果暗示tan可能是基于一种转基因分析,这种分析可能会对涉及进化变化的基因产生误导性结果。
Previous work on Drosophila santomea suggested that its absence of abdominal pigmentation, compared to the other darkly-pigmented species, is based on mutations in the cis-regulatory region of tan inactivating the expression of that gene in the abdomen of D. santomea males and females. Our discovery that D. santomea males can produce viable hybrids when mated to D. melanogaster females enables us to use the armamentarium of genetic tools in the latter species to study the genetic basis of this interspecific difference in pigmentation. Hybridization tests using D. melanogaster deficiencies that include tan show no evidence that this locus is involved in the lighter pigmentation of D. santomea females; rather, the pigmentation difference appears to involve at least four other loci in the region. Earlier results implicating tan may have been based on a type of transgenic analysis that can give misleading results about the genes involved in an evolutionary change.
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