Neutralization of SARS-CoV-2 lineage B.1.1.7 pseudovirus by BNT162b2 vaccine-elicited human sera.
Neutralization of SARS-CoV-2 lineage B.1.1.7 pseudovirus by BNT162b2 vaccine-elicited human sera.
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DOI：
10.1126/science.abg6105
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发表时间：
2021-03-12
期刊：
影响因子：
--
通讯作者：
Şahin U
中科院分区：
文献类型：
--
作者：
Muik A;Wallisch AK;Sänger B;Swanson KA;Mühl J;Chen W;Cai H;Maurus D;Sarkar R;Türeci Ö;Dormitzer PR;Şahin U
2020年底在英国出现的严重急性呼吸综合征冠状病毒2 (SARS-CoV-2) B1.1.7 （VOC 2012012 /01）变体在刺突蛋白基因上发生了许多变化。其中三种与增强的传染性和传播性有关，人们担心B.1.1.7可能会损害疫苗的有效性。Muik等人比较了40名接种BioNTech-Pfizer mRNA疫苗BNT162b2的受试者血清对携带武汉参考毒株或B.1.1.7刺突蛋白的假病毒的中和效果（见Altmann等人的观点）。在接种强化疫苗21天后，从两个年龄组的40名受试者中提取血清。疫苗对B.1.1.7仍然有效，中和作用略有但显著下降，在55岁以下的参与者中更为明显。因此，该疫苗对这种变异提供了重要的“缓冲”保护。《科学》，本期第1152页；另见p.尽管B.1.1.7 (VOC 2012012 /01) 2020英国SARS-CoV-2变体存在许多遗传变化，但BioNTech-Pfizer mRNA疫苗仍具有保护作用。最近，在英国出现了一种新的严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）谱系，称为B.1.1.7（关注变体：VOC 2012012 /01），据报道，它比其他菌株传播得更有效、更快。这种变异具有异常大量的突变，在刺突(S)蛋白中有10个氨基酸发生变化，这引起了人们对中和抗体识别可能受到影响的担忧。在这项研究中，我们用40名参与者的血清检测了携带武汉参考毒株或B.1.1.7谱系刺突蛋白的SARS-CoV-2-S假病毒，这些参与者在先前报道的一项试验中接种了基于信使rna的COVID-19疫苗BNT162b2。免疫血清对B.1.1.7假病毒的中和效价略有降低，但总体上基本保持不变。这些数据表明B.1.1.7谱系不会逃脱bnt162b2介导的保护。
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) B1.1.7 (VOC 202012/01) variant that emerged in late 2020 in the United Kingdom has many changes in the spike protein gene. Three of these are associated with enhanced infectivity and transmissibility, and there are concerns that B.1.1.7 might compromise the effectiveness of the vaccine. Muik et al. compared the neutralization efficacy of sera from 40 subjects immunized with the BioNTech-Pfizer mRNA vaccine BNT162b2 against a pseudovirus bearing the Wuhan reference strain or the lineage B.1.1.7 spike protein (see the Perspective by Altmann et al.). Serum was derived from 40 subjects in two age groups 21 days after the booster shot. The vaccine remained effective against B.1.1.7 with a slight but significant decrease in neutralization that was more apparent in participants under 55 years of age. Thus, the vaccine provides a significant “cushion” of protection against this variant. Science, this issue p. 1152; see also p. Despite the many genetic changes in the B.1.1.7 (VOC 202012/01) 2020 UK variant of SARS-CoV-2, the BioNTech-Pfizer mRNA vaccine remains protective. Recently, a new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineage called B.1.1.7 (variant of concern: VOC 202012/01), which is reported to spread more efficiently and faster than other strains, emerged in the United Kingdom. This variant has an unusually large number of mutations, with 10 amino acid changes in the spike (S) protein, raising concerns that its recognition by neutralizing antibodies may be affected. In this study, we tested SARS-CoV-2-S pseudoviruses bearing either the Wuhan reference strain or the B.1.1.7 lineage spike protein with sera of 40 participants who were vaccinated in a previously reported trial with the messenger RNA–based COVID-19 vaccine BNT162b2. The immune sera had slightly reduced but overall largely preserved neutralizing titers against the B.1.1.7 lineage pseudovirus. These data indicate that the B.1.1.7 lineage will not escape BNT162b2-mediated protection.
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BNT162B2 mRNA COVID-19疫苗的安全性和功效。
DOI：
10.1056/nejmoa2034577
发表时间：
2020-12-31
期刊：
The New England journal of medicine
影响因子：
--
作者：
Polack FP;Thomas SJ;Kitchin N;Absalon J;Gurtman A;Lockhart S;Perez JL;Pérez Marc G;Moreira ED;Zerbini C;Bailey R;Swanson KA;Roychoudhury S;Koury K;Li P;Kalina WV;Cooper D;Frenck RW Jr;Hammitt LL;Türeci Ö;Nell H;Schaefer A;Ünal S;Tresnan DB;Mather S;Dormitzer PR;Şahin U;Jansen KU;Gruber WC;C4591001 Clinical Trial Group
通讯作者：
C4591001 Clinical Trial Group
DOI：
10.1126/science.abc4730
发表时间：
2020-09-25
期刊：
Science (New York, N.Y.)
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