Fine structural tuning of the assembly of ECM peptide conjugates via slight sequence modifications.
Fine structural tuning of the assembly of ECM peptide conjugates via slight sequence modifications.
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DOI：
10.1126/sciadv.abd3033
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发表时间：
2020-10
期刊：
影响因子：
13.6
通讯作者：
Kiick KL
中科院分区：
文献类型：
--
作者：
Qin J;Sloppy JD;Kiick KL
可以改变基于肽的纳米载体的热响应性和结构，以实现多种货物输送机制。弹性蛋白样肽和类胶原蛋白肽 (ELP-CLP) 缀合物的纳米结构自组装已被研究作为生产热响应性胶原蛋白结合药物输送载体的方法。受我们之前的工作启发，其中ELP-CLP缀合物成功地自组装成囊泡和血小板状纳米结构，在这里，我们将我们的ELP-CLP生物缀合物库扩展到一系列具有不同结构域长度的含有色氨酸/苯丙氨酸的ELP和基于GPO的CLP [W2Fx-b-(GPO)y]，以确定这些修饰对热响应性和形态的影响。具有较长 ELP 或 CLP 结构域的缀合物的较低转变温度能够在接近生理温度的情况下形成明确的纳米颗粒。此外，当 ELP/CLP 长度比减小时，会发生从囊泡到片状纳米结构的形态转变。鉴于先前已证明许多 ELP-CLP 生物缀合物与疏水性药物和含胶原材料结合的能力，我们的结果表明为靶向生物应用设计特定的热响应纳米结构提供了新的机会。
The thermoresponsiveness and structures of peptide-based nanocarriers can be altered to enable versatile mechanisms of cargo delivery. The self-assembly of nanostructures from conjugates of elastin-like peptides and collagen-like peptides (ELP-CLP) has been studied as means to produce thermoresponsive, collagen-binding drug delivery vehicles. Motivated by our previous work in which ELP-CLP conjugates successfully self-assembled into vesicles and platelet-like nanostructures, here, we extend our library of ELP-CLP bioconjugates to a series of tryptophan/phenylalanine-containing ELPs and GPO-based CLPs [W2Fx-b-(GPO)y] with various domain lengths to determine the impact of these modifications on the thermoresponsiveness and morphology. The lower transition temperature of the conjugates with longer ELP or CLP domains enables the formation of well-defined nanoparticles near physiological temperature. Moreover, the morphological transition from vesicles to platelet-like nanostructures occurred when the ratio of the lengths of ELP/CLP decreased. Given the previously demonstrated ability of many ELP-CLP bioconjugates to bind to both hydrophobic drugs and collagen-containing materials, our results suggest new opportunities for designing specific thermoresponsive nanostructures for targeted biological applications.
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影响因子：
17.1
作者：
Luo, Jingnan;Tong, Yen Wah
通讯作者：
Tong, Yen Wah
影响因子：
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作者：
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15
作者：
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Raines, RT
影响因子：
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Dunshee, Lucas C.;Sullivan, Millicent O.;Kiick, Kristi L.
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Kiick, Kristi L.
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影响因子：
15
作者：
Du, Jin-Zhi;Du, Xiao-Jiao;Wang, Jun
通讯作者：
Wang, Jun