The influence of HMF and furfural on redox-balance and energy-state of xylose-utilizing Saccharomyces cerevisiae.
The influence of HMF and furfural on redox-balance and energy-state of xylose-utilizing Saccharomyces cerevisiae.
复制标题
DOI：
10.1186/1754-6834-6-22
复制
发表时间：
2013-02-15
影响因子：
6.3
通讯作者：
Olsson L
中科院分区：
文献类型：
--
作者：
Ask M;Bettiga M;Mapelli V;Olsson L
木质纤维素乙醇生产的预处理生物质产生的化合物可以抑制微生物代谢。呋喃醛类羟甲基糠醛（HMF）和糠醛近年来受到越来越多的关注。在本研究中，研究了HMF和糠醛在厌氧趋化器中对氧化还原代谢、能量代谢和基因表达的影响。通过对利用木糖的酿酒酵母VTT C-10883在HMF和糠醛存在下的培养，发现在呋喃醛存在下的细胞内氧化还原辅助因子浓度、分解代谢和合成代谢还原电荷明显低于无抑制剂的培养。当HMF和糠醛存在时，分解代谢还原电荷从0.13（±0.005）降低到0.08（±0.002），合成代谢还原电荷从0.46（±0.11）降低到0.27（±0.02）。当添加抑制剂时，细胞内ATP浓度较低，但与对照组相比，能量电荷仅从0.87（±0.002）下降到0.85（±0.004）。转录组分析和上调基因的MIPS功能富集分析显示，与对照培养相比，当抑制剂存在时，“细胞拯救、防御和毒力”功能组被过度代表。其中，ATP结合外排泵PDR5和YOR1被认为对抑制剂外排很重要，可能是应激细胞内ATP浓度较低的原因。研究还发现，当饲料培养基中存在氮饥饿抑制剂时，与假菌丝生长有关的基因是上调最多的。参与氨基酸代谢、乙醛酸循环、电子传递和氨基酸运输的基因在响应HMF和糠醛的下调基因集中富集。推测HMF和糠醛诱导的NADPH排水可能影响氨同化，从而以假菌丝生长和氨基酸合成下调的形式引起氮饥饿反应。HMF和糠醛严重影响了氧化还原代谢，而对能量代谢的影响不太明显，这表明氧化还原系统的工程设计是开发更健壮的生物乙醇生产菌株的可能策略。
Pretreatment of biomass for lignocellulosic ethanol production generates compounds that can inhibit microbial metabolism. The furan aldehydes hydroxymethylfurfural (HMF) and furfural have received increasing attention recently. In the present study, the effects of HMF and furfural on redox metabolism, energy metabolism and gene expression were investigated in anaerobic chemostats where the inhibitors were added to the feed-medium. By cultivating the xylose-utilizing Saccharomyces cerevisiae strain VTT C-10883 in the presence of HMF and furfural, it was found that the intracellular concentrations of the redox co-factors and the catabolic and anabolic reduction charges were significantly lower in the presence of furan aldehydes than in cultivations without inhibitors. The catabolic reduction charge decreased from 0.13(±0.005) to 0.08(±0.002) and the anabolic reduction charge decreased from 0.46(±0.11) to 0.27(±0.02) when HMF and furfural were present. The intracellular ATP concentration was lower when inhibitors were added, but resulted only in a modest decrease in the energy charge from 0.87(±0.002) to 0.85(±0.004) compared to the control. Transcriptome profiling followed by MIPS functional enrichment analysis of up-regulated genes revealed that the functional group “Cell rescue, defense and virulence” was over-represented when inhibitors were present compared to control cultivations. Among these, the ATP-binding efflux pumps PDR5 and YOR1 were identified as important for inhibitor efflux and possibly a reason for the lower intracellular ATP concentration in stressed cells. It was also found that genes involved in pseudohyphal growth were among the most up-regulated when inhibitors were present in the feed-medium suggesting nitrogen starvation. Genes involved in amino acid metabolism, glyoxylate cycle, electron transport and amino acid transport were enriched in the down-regulated gene set in response to HMF and furfural. It was hypothesized that the HMF and furfural-induced NADPH drainage could influence ammonia assimilation and thereby give rise to the nitrogen starvation response in the form of pseudohyphal growth and down-regulation of amino acid synthesis. The redox metabolism was severely affected by HMF and furfural while the effects on energy metabolism were less evident, suggesting that engineering of the redox system represents a possible strategy to develop more robust strains for bioethanol production.
登录
查看更多内容
影响因子：
8.4
作者：
Hou, Jin;Lages, Nuno F.;Vemuri, Goutham N.
通讯作者：
Vemuri, Goutham N.
影响因子：
6.3
作者：
Allen SA;Clark W;McCaffery JM;Cai Z;Lanctot A;Slininger PJ;Liu ZL;Gorsich SW
通讯作者：
Gorsich SW
影响因子：
4.8
作者：
DeLuna, A;Avendaño, A;González, A
通讯作者：
González, A
DOI：
10.1111/j.1749-6632.1986.tb26517.x
发表时间：
1986-05-22
影响因子：
5.2
作者：
FIREOVED, RL;MUTHARASAN, R
通讯作者：
MUTHARASAN, R
影响因子：
5
作者：
Almeida, Joao R. M.;Bertilsson, Magnus;Gorwa-Grauslund, Marie-F.
通讯作者：
Gorwa-Grauslund, Marie-F.