A sustained release of BMP2 in urine-derived stem cells enhances the osteogenic differentiation and the potential of bone regeneration.
A sustained release of BMP2 in urine-derived stem cells enhances the osteogenic differentiation and the potential of bone regeneration.
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尿源干细胞中持续释放 BMP2 可增强成骨分化和骨再生潜力
DOI：
10.1093/rb/rbac015
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发表时间：
2022
影响因子：
6.7
通讯作者：
Xiang, Zhou
中科院分区：
文献类型：
--
作者：
Wu, Shuang;Chen, Zhao;Yu, Xi;Duan, Xin;Chen, Jialei;Liu, Guoming;Gong, Min;Xing, Fei;Sun, Jiachen;Huang, Shishu;Xiang, Zhou
以细胞为基础的组织工程是替代目前骨缺损治疗的最乐观的方法之一。尿源性干细胞（USCs）是非侵入性获得的，成为骨再生的有前途的种子细胞之一。制备了一种可注射的骨形成蛋白2壳聚糖微球/I型胶原水凝胶（BMP 2-CSM/Col I hydrogel）。USCs以时间依赖性的方式增殖，以良好的延伸性扩散，并且在2D和3D模型的不同水凝胶中彼此互连。BMP 2以持续模式释放超过28天。缓释BMP-2可增加2D培养USCs的ALP活性和矿物质沉积，促进3D培养USCs成骨相关基因和蛋白的表达。在体内，USCs与BMP 2-CSM/Col I水凝胶的混合物有效地促进了骨再生，植入8周后新骨体积占总骨体积的比例为38%。结果表明，BMP 2-CSM/Col I水凝胶在体外2D和3D培养中促进了USCs的成骨分化，为体内骨组织工程提供了一种有前途的细胞来源。因此，USCs接种的水凝胶支架被认为是骨缺损修复的替代方法。
Cell-based tissue engineering is one of the optimistic approaches to replace current treatments for bone defects. Urine-derived stem cells (USCs) are obtained non-invasively and become one of the promising seed cells for bone regeneration. An injectable BMP2-releasing chitosan microspheres/type I collagen hydrogel (BMP2-CSM/Col I hydrogel) was fabricated. USCs proliferated in a time-dependent fashion, spread with good extension and interconnected with each other in different hydrogels both for 2D and 3D models. BMP2 was released in a sustained mode for more than 28 days. Sustained-released BMP2 increased the ALP activities and mineral depositions of USCs in 2D culture, and enhanced the expression of osteogenic genes and proteins in 3D culture. In vivo, the mixture of USCs and BMP2-CSM/Col I hydrogels effectively enhanced bone regeneration, and the ratio of new bone volume to total bone volume was 38% after 8 weeks of implantation. Our results suggested that BMP2-CSM/Col I hydrogels promoted osteogenic differentiation of USCs in 2D and 3D culture in vitro and USCs provided a promising cell source for bone tissue engineering in vivo. As such, USCs-seeded hydrogel scaffolds are regarded as an alternative approach in the repair of bone defects.
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影响因子：
7.5
作者：
Marolt D;Knezevic M;Novakovic GV
通讯作者：
Novakovic GV
基于组装壳聚糖微球的具有再生和抗感染双重功效的注射凝胶结构
影响因子：
9.5
作者：
Cai, Bin;Zou, Qin;Li, Yubao
通讯作者：
Li, Yubao
DOI：
10.1016/j.msec.2021.112207
发表时间：
2021-06-11
影响因子：
7.9
作者：
Diallo, Ahmad Moustapha;Rota, Solene;Anagnostou, Fani
通讯作者：
Anagnostou, Fani
影响因子：
14
作者：
Guan, Junjie;Zhang, Jieyuan;Chang, Jiang
通讯作者：
Chang, Jiang
影响因子：
10
作者：
Jiang, Tongmeng;Xu, Guojie;Zheng, Li
通讯作者：
Zheng, Li