The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic.
The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic.
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DOI：
10.1083/jcb.200607151
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发表时间：
2007-01-29
期刊：
影响因子：
--
通讯作者：
Munro S
中科院分区：
文献类型：
--
作者：
Behnia R;Barr FA;Flanagan JJ;Barlowe C;Munro S
哺乳动物高尔基体蛋白GRASP 65在重建脑池堆积和囊泡束缚的测定中是必需的。通过N-末端肉豆蔻酰基连接到膜上，它招募卷曲螺旋蛋白GM 130。该系统与芽殖酵母的相关性尚不清楚，因为它们缺乏GM 130的明显直系同源物，并且它们唯一的GRASP 65亲属（Grh 1）缺乏豆蔻酰化位点，甚至被认为在有丝分裂检查点中起作用。在这项研究中，我们表明，Grh 1有一个N-末端的两亲性螺旋，是N-末端乙酰化和介导协会与顺式高尔基体。我们发现，Grh 1形成了一个复杂的与以前未知的卷曲螺旋蛋白，Ydl 099 w（Bug 1）。此外，Grh 1与COPII涂层的Sec 23/24组分相互作用。Grh 1和Bug 1都不是生长所必需的，但生化测定和与已知的囊泡束缚介质（Uso 1和Ypt 1）的遗传相互作用表明，Grh 1-Bug 1复合物有助于形成冗余的相互作用网络，介导COPII囊泡的消耗和顺式高尔基体的形成。
The mammalian Golgi protein GRASP65 is required in assays that reconstitute cisternal stacking and vesicle tethering. Attached to membranes by an N-terminal myristoyl group, it recruits the coiled-coil protein GM130. The relevance of this system to budding yeasts has been unclear, as they lack an obvious orthologue of GM130, and their only GRASP65 relative (Grh1) lacks a myristoylation site and has even been suggested to act in a mitotic checkpoint. In this study, we show that Grh1 has an N-terminal amphipathic helix that is N-terminally acetylated and mediates association with the cis-Golgi. We find that Grh1 forms a complex with a previously uncharacterized coiled-coil protein, Ydl099w (Bug1). In addition, Grh1 interacts with the Sec23/24 component of the COPII coat. Neither Grh1 nor Bug1 are essential for growth, but biochemical assays and genetic interactions with known mediators of vesicle tethering (Uso1 and Ypt1) suggest that the Grh1–Bug1 complex contributes to a redundant network of interactions that mediates consumption of COPII vesicles and formation of the cis-Golgi.
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影响因子：
21.3
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