Expression of S100A6 in rat hippocampus after traumatic brain injury due to lateral head acceleration.
Expression of S100A6 in rat hippocampus after traumatic brain injury due to lateral head acceleration.
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侧向头部加速性脑损伤大鼠海马中S100A6的表达
DOI：
10.3390/ijms15046378
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发表时间：
2014-04-15
影响因子：
5.6
通讯作者：
Huang JH
中科院分区：
文献类型：
--
作者：
Fang B;Liang M;Yang G;Ye Y;Xu H;He X;Huang JH
在创伤性脑损伤 (TBI) 大鼠模型中，我们研究了海马认知功能和 S100A6 表达的变化。此前尚未报道过该蛋白与 TBI 相关的变化。在侧头加速或假手术后，通过免疫组织化学染色、蛋白质印迹和逆转录聚合酶链反应 (RT-PCR) 对大鼠 S100A6 进行研究。 TBI 后 1 小时观察到 S100A6 蛋白和 mRNA 水平降低，随后在 6、12、24 和 72 小时内逐渐增加，然后在 14 天时恢复至假手术水平。莫里斯水迷宫（MWM）测试用于评估动物的空间认知。 TBI 大鼠和假大鼠表现出明显的学习曲线，表示为逃避潜伏期。尽管 TBI 大鼠的认知能力比假手术大鼠相对较差，但这种差异在损伤后早期并不显着。与假手术动物相比，受伤后 72 小时观察到 TBI 大鼠明显的认知缺陷。 TBI 大鼠在每日 MWM 测试中表现出平台穿越次数减少；伤后72小时的表现最差。总之，S100A6的降低可能是TBI后导致继发性认知下降的早期事件之一，其随后的升高与认知改善密切相关。 S100A6可能在TBI神经元变性和再生中发挥重要作用。
In a rat model of traumatic brain injury (TBI), we investigated changes in cognitive function and S100A6 expression in the hippocampus. TBI-associated changes in this protein have not previously been reported. Rat S100A6 was studied via immunohistochemical staining, Western blot, and reverse transcription-polymerase chain reaction (RT-PCR) after either lateral head acceleration or sham. Reduced levels of S100A6 protein and mRNA were observed 1 h after TBI, followed by gradual increases over 6, 12, 24, and 72 h, and then a return to sham level at 14 day. Morris water maze (MWM) test was used to evaluate animal spatial cognition. TBI- and sham-rats showed an apparent learning curve, expressed as escape latency. Although TBI-rats displayed a relatively poorer cognitive ability than sham-rats, the disparity was not significant early post-injury. Marked cognitive deficits in TBI-rats were observed at 72 h post-injury compared with sham animals. TBI-rats showed decreased times in platform crossing in the daily MWM test; the performance at 72 h post-injury was the worst. In conclusion, a reduction in S100A6 may be one of the early events that lead to secondary cognitive decline after TBI, and its subsequent elevation is tightly linked with cognitive improvement. S100A6 may play important roles in neuronal degeneration and regeneration in TBI.
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影响因子：
3.3
作者：
Hoskison MM;Moore AN;Hu B;Orsi S;Kobori N;Dash PK
通讯作者：
Dash PK
影响因子：
4.8
作者：
Leclerc, Estelle;Fritz, Gunter;Galichet, Arnaud
通讯作者：
Galichet, Arnaud
影响因子：
4.1
作者：
DEARDEN, NM;GIBSON, JS;CAMERON, MM
通讯作者：
CAMERON, MM
影响因子：
4.8
作者：
Courtois-Coutry, N;Le Moellic, C;Blot-Chabaud, M
通讯作者：
Blot-Chabaud, M
影响因子：
3.7
作者：
Douma, BRK;Korte, SM;Luiten, PGM
通讯作者：
Luiten, PGM