Impact of heating on passive and active biomechanics of suspended cells.
Impact of heating on passive and active biomechanics of suspended cells.
复制标题
DOI：
10.1098/rsfs.2013.0069
复制
发表时间：
2014-04-06
期刊：
影响因子：
4.4
通讯作者：
Guck J
中科院分区：
文献类型：
--
作者：
Chan CJ;Whyte G;Boyde L;Salbreux G;Guck J
细胞是一种复杂的材料，其机械性能对其正常功能至关重要。加热可以对这些机械性能产生巨大影响，类似于其对人工聚合物网络动力学的影响。我们研究了这样的机械变化，通过使用微流体光学拉伸器，这使我们能够探测细胞力学时，细胞在不同的时间尺度受到不同的加热条件。我们发现，HL 60/S4髓样前体细胞变得机械更顺应性和流体样时，受到突然的激光诱导的温度升高或长期暴露于更高的环境温度。在52 ± 1°C的临界温度以上，我们观察到活跃的细胞收缩，这与通过温度敏感的瞬时受体电位香草酸2（TRPV 2）离子通道的钙内流密切相关，随后细胞体积膨胀。从被动到主动的细胞反应的变化可以有效地描述了一个力学模型，包括主动应力和粘弹性组件。我们的工作突出了TRPV 2在调节细胞热机械反应中的作用。它还提供了深入了解皮层张力和渗透压如何支配细胞力学和调节细胞形状变化，以响应热和机械应力。
A cell is a complex material whose mechanical properties are essential for its normal functions. Heating can have a dramatic effect on these mechanical properties, similar to its impact on the dynamics of artificial polymer networks. We investigated such mechanical changes by the use of a microfluidic optical stretcher, which allowed us to probe cell mechanics when the cells were subjected to different heating conditions at different time scales. We find that HL60/S4 myeloid precursor cells become mechanically more compliant and fluid-like when subjected to either a sudden laser-induced temperature increase or prolonged exposure to higher ambient temperature. Above a critical temperature of 52 ± 1°C, we observed active cell contraction, which was strongly correlated with calcium influx through temperature-sensitive transient receptor potential vanilloid 2 (TRPV2) ion channels, followed by a subsequent expansion in cell volume. The change from passive to active cellular response can be effectively described by a mechanical model incorporating both active stress and viscoelastic components. Our work highlights the role of TRPV2 in regulating the thermomechanical response of cells. It also offers insights into how cortical tension and osmotic pressure govern cell mechanics and regulate cell-shape changes in response to heat and mechanical stress.
登录
查看更多内容
影响因子：
3.4
作者：
Alcaraz, J;Buscemi, L;Navajas, D
通讯作者：
Navajas, D
影响因子：
3.8
作者：
Ebert, Susanne;Travis, Kort;Guck, Jochen
通讯作者：
Guck, Jochen
DOI：
10.1364/josaa.26.001814
发表时间：
2009-08-01
影响因子：
1.9
作者：
Boyde, Lars;Chalut, Kevin J.;Guck, Jochen
通讯作者：
Guck, Jochen
DOI：
10.1073/pnas.0811261106
发表时间：
2009-09-15
影响因子：
11.1
作者：
Lautenschlaeger, Franziska;Paschke, Stephan;Guck, Jochen
通讯作者：
Guck, Jochen
DOI：
10.1073/pnas.96.17.9602
发表时间：
1999-08-17
影响因子：
11.1
作者：
Kato, H;Nishizaka, T;Ishiwata, S
通讯作者：
Ishiwata, S