Genetic Regulation of the 2D to 3D Growth Transition in the Moss Physcomitrella patens.
Genetic Regulation of the 2D to 3D Growth Transition in the Moss Physcomitrella patens.
复制标题
DOI：
10.1016/j.cub.2017.12.052
复制
发表时间：
2018-02-05
期刊：
影响因子：
--
通讯作者：
Langdale JA
中科院分区：
文献类型：
--
作者：
Moody LA;Kelly S;Rabbinowitsch E;Langdale JA
地球生命史上最重要的事件之一是植物对土地的殖民化;这一转变与三维（3D）生长的进化相吻合，并且很可能是由三维（3D）生长的进化实现的。今天，在陆地生物圈中表现出的多样形态来自于发育过程中3D生长过程的差异调节。在许多植物中，3D生长在合子的最初几次分裂期间开始，因此，遗传基础不能被解剖，因为突变体不能存活。然而，在作为最早陆地植物代表的苔藓中，3D芽生长之前是可以无限期维持的2D丝状阶段。在这里，我们使用苔藓小立碗藓来鉴定2D到3D转变的遗传调节因子。突变筛选产生了只能在2D中生长的个体，通过将体细胞杂交与批量分离分析和基因组测序相结合的创新策略，在其中一个中鉴定了致病突变。NO GAMETOPHORES 1（NOG 1）基因编码一种泛素相关蛋白，仅存在于陆地植物基因组中。在缺乏PpNOG 1功能的突变体中，编码3D促进PpAPB转录因子的转录物显著减少，并且不形成指定用于3D生长的顶端初始细胞。PpNOG 1在2D到3D转变的最早确定阶段起作用，可能通过降解抑制3D生长的蛋白质。因此，在陆地植物中获得NOG 1功能可能使3D形态学的进化和发展成为可能。NO GAMETOPHORES 1（PpNOG 1）调控小垂苔2D向3D的生长转变PpNOG 1作用于3D促进PpAPB转录因子的上游PpNOG 1是3D生长所需的顶端初始细胞的形成所必需的NOG 1基因仅在陆地植物中发现，因此与3D生长同步进化Moody et al.使用正向遗传学、体细胞杂交和基因组测序来鉴定NO GAMETOPHORES 1（PpNOG 1），这是一种调节苔藓小立碗藓（Physcomitrella patens）中二维（2D）到三维（3D）生长转变的基因。PpNOG 1促进建立3D生长所需的顶端原始细胞的形成。
One of the most important events in the history of life on earth was the colonization of land by plants; this transition coincided with and was most likely enabled by the evolution of 3-dimensional (3D) growth. Today, the diverse morphologies exhibited across the terrestrial biosphere arise from the differential regulation of 3D growth processes during development. In many plants, 3D growth is initiated during the first few divisions of the zygote, and therefore, the genetic basis cannot be dissected because mutants do not survive. However, in mosses, which are representatives of the earliest land plants, 3D shoot growth is preceded by a 2D filamentous phase that can be maintained indefinitely. Here, we used the moss Physcomitrella patens to identify genetic regulators of the 2D to 3D transition. Mutant screens yielded individuals that could only grow in 2D, and through an innovative strategy that combined somatic hybridization with bulk segregant analysis and genome sequencing, the causative mutation was identified in one of them. The NO GAMETOPHORES 1 (NOG1) gene, which encodes a ubiquitin-associated protein, is present only in land plant genomes. In mutants that lack PpNOG1 function, transcripts encoding 3D-promoting PpAPB transcription factors are significantly reduced, and apical initial cells specified for 3D growth are not formed. PpNOG1 acts at the earliest identified stage of the 2D to 3D transition, possibly through degradation of proteins that suppress 3D growth. The acquisition of NOG1 function in land plants could thus have enabled the evolution and development of 3D morphology. NO GAMETOPHORES 1 (PpNOG1) regulates the 2D to 3D growth transition in P. patens PpNOG1 acts upstream of 3D-promoting PpAPB transcription factors PpNOG1 is required for the formation of apical initial cells specified for 3D growth NOG1 genes are found only in land plants and thus evolved coincident with 3D growth Moody et al. use forward genetics, somatic hybridization, and genome sequencing to identify NO GAMETOPHORES 1 (PpNOG1), a gene that regulates the two-dimensional (2D) to three-dimensional (3D) growth transition in the moss Physcomitrella patens. PpNOG1 promotes the formation of apical initials that are required for the establishment of 3D growth.
登录
查看更多内容
DOI：
10.1111/nph.14318
发表时间：
2017-10
期刊：
The New phytologist
影响因子：
--
作者：
Frangedakis E;Saint-Marcoux D;Moody LA;Rabbinowitsch E;Langdale JA
通讯作者：
Langdale JA
影响因子：
7.4
作者：
Demko, Viktor;Perroud, Pierre-Francois;Olsen, Odd-Arne
通讯作者：
Olsen, Odd-Arne
DOI：
10.1007/bf00265582
发表时间：
1977-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
GRIMSLEY, NH;ASHTON, NW;COVE, DJ
通讯作者：
COVE, DJ
影响因子：
9.2
作者：
Prigge, Michael J.;Lavy, Meirav;Estelle, Mark
通讯作者：
Estelle, Mark
影响因子：
10.7
作者：
Nguyen LT;Schmidt HA;von Haeseler A;Minh BQ
通讯作者：
Minh BQ