Impact of acute inflammation on spinal motoneuron synaptic plasticity following ventral root avulsion.
Impact of acute inflammation on spinal motoneuron synaptic plasticity following ventral root avulsion.
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DOI：
10.1186/1742-2094-7-29
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发表时间：
2010-05-04
影响因子：
9.3
通讯作者：
Oliveira AL
中科院分区：
文献类型：
--
作者：
Barbizan R;Oliveira AL
腹根撕脱伤是一种近端神经根病变，其中腹运动神经根从脊髓表面撕裂，导致运动神经元广泛死亡。先前的研究表明，如果在患有实验性自身免疫性脑脊髓炎（EAE）的动物中进行这种损伤，尽管存在强烈的炎症反应，但仍可以挽救大量运动神经元。这种救援效应归因于入侵的 T 细胞产生了许多神经营养因子。突触变化可能与神经元变性有关，更好地了解这些变化的作用对于制定促进神经元存活的新策略可能很重要。目前工作的目的是评估 EAE 动物腹根撕脱后脊髓前角运动核的神经元存活、星形胶质细胞反应和突触输入变化，无论是在疾病高峰期还是缓解后。对Lewis大鼠进行单侧腰椎腹侧根撕脱术（VRA），并将其分为三组：VRA对照组、EAE高峰时的VRA和EAE缓解期间的VRA。处死动物并对其腰脊髓进行免疫组织化学、透射电子显微镜和运动神经元计数处理。结果表明，与单独使用 VRA 相比，VRA+EAE 组中星形胶质细胞反应减少，小胶质细胞反应性得以维持，运动神经元的突触覆盖和存活率增加。目前的研究结果表明，中枢神经系统炎症可能直接影响突触可塑性以及神经元网络的稳定性，从而对受损神经元的存活产生积极影响。
Ventral root avulsion is a proximal nerve root lesion in which ventral motor nerve rootlets are torn from surface of the spinal cord, resulting in extensive death of motoneurons. It has been previously shown that if such lesioning is performed in an animal with experimental autoimmune encephalomyelitis (EAE), a significant number of motoneurons can be rescued despite an intense inflammatory reaction. This rescue effect has been attributed to production of a number of neurotrophic factors by invading T cells. Synaptological changes may be involved in neuronal degeneration, and a better understanding of the role of these changes may be of importance for developing new strategies to promote neuronal survival. The objective of the present work was to evaluate neuronal survival, astroglial reaction and synaptic input changes in spinal cord anterior horn motor nuclei after ventral root avulsion in animals with EAE, both during peak disease and after remission. Lewis rats were subjected to unilateral avulsion of lumbar ventral roots (VRA) and divided into three groups: VRA control, VRA at peak of EAE, and VRA during EAE remission. The animals were sacrificed and their lumbar spinal cords processed for immunohistochemistry, transmission electron microscopy, and motoneuron counting. The results indicate a reduction in astroglial reaction, a maintenance of microglial reactivity, and increases in synaptic covering of, and survival of, motoneurons in the VRA+EAE group as compared to VRA alone. The present findings indicate that CNS inflammation may directly influence synaptic plasticity as well as the stability of neuronal networks, positively influencing the survival of lesioned neurons.
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影响因子：
2.5
作者：
Bulloch, Karen;Miller, Melinda M.;McEwen, Bruce S.
通讯作者：
McEwen, Bruce S.
影响因子：
6.2
作者：
GEHRMANN, J;GOLD, R;KREUTZBERG, GW
通讯作者：
KREUTZBERG, GW
影响因子：
3.4
作者：
Marques, K. B.;Santos, L. M. B.;Oliveira, A. L. R.
通讯作者：
Oliveira, A. L. R.
影响因子：
3.4
作者：
PIEHL, F;ARVIDSSON, U;HOKFELT, T
通讯作者：
HOKFELT, T
影响因子：
25
作者：
Essrich, C;Lorez, M;Lüscher, B
通讯作者：
Lüscher, B