Genetic and Chemical Engineering of Phages for Controlling Multidrug-Resistant Bacteria.
Genetic and Chemical Engineering of Phages for Controlling Multidrug-Resistant Bacteria.
复制标题
控制多重耐药细菌的噬菌体基因与化学工程
DOI：
10.3390/antibiotics10020202
复制
发表时间：
2021-02-19
期刊：
影响因子：
--
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Guo D;Chen J;Zhao X;Luo Y;Jin M;Fan F;Park C;Yang X;Sun C;Yan J;Chen W;Liu Z
沿着抗生素的过度使用，多重耐药菌的出现和传播已成为公共卫生问题，对细菌感染的控制和治疗构成巨大挑战。作为细菌的天敌，细菌的研究重新引起了人们的关注。噬菌体治疗被认为是后抗生素时代对抗病原体的最有前途的替代策略之一。近年来，基因工程和化学工程方法已被应用于噬菌体的改造。其中，基因工程包括毒素蛋白的表达、宿主识别受体的修饰和细菌噬菌体抗性途径的干扰。同时，化学工程涉及用抗生素、抗菌肽、重金属离子和光热物质交联噬菌体外壳。这些进展极大地扩大了噬菌体的宿主范围，提高了其杀菌效率，这为噬菌体治疗在控制多重耐药病原体中的应用提供了新的思路。这篇综述报道了通过遗传和化学方法进行工程改造的方法。此外，我们提出了这种新型抗菌剂所遇到的障碍。
Along with the excessive use of antibiotics, the emergence and spread of multidrug-resistant bacteria has become a public health problem and a great challenge vis-à-vis the control and treatment of bacterial infections. As the natural predators of bacteria, phages have reattracted researchers’ attentions. Phage therapy is regarded as one of the most promising alternative strategies to fight pathogens in the post-antibiotic era. Recently, genetic and chemical engineering methods have been applied in phage modification. Among them, genetic engineering includes the expression of toxin proteins, modification of host recognition receptors, and interference of bacterial phage-resistant pathways. Chemical engineering, meanwhile, involves crosslinking phage coats with antibiotics, antimicrobial peptides, heavy metal ions, and photothermic matters. Those advances greatly expand the host range of phages and increase their bactericidal efficiency, which sheds light on the application of phage therapy in the control of multidrug-resistant pathogens. This review reports on engineered phages through genetic and chemical approaches. Further, we present the obstacles that this novel antimicrobial has incurred.
登录
查看更多内容
影响因子：
46.9
作者：
Bikard, David;Euler, Chad W.;Jiang, Wenyan;Nussenzweig, Philip M.;Goldberg, Gregory W.;Duportet, Xavier;Fischetti, Vincent A.;Marraffini, Luciano A.
通讯作者：
Marraffini, Luciano A.
DOI：
10.3390/antibiotics7030066
发表时间：
2018-07-27
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Domingo-Calap P;Delgado-Martínez J
通讯作者：
Delgado-Martínez J
大肠杆菌对恩诺沙星的耐药和耐受机制分析
影响因子：
3
作者：
Bai, Hua;Du, Jia-fa;Liu, Yu-qing
通讯作者：
Liu, Yu-qing
影响因子：
16
作者：
Dolan, Adam E.;Hou, Zhonggang;Zhang, Yan
通讯作者：
Zhang, Yan
影响因子：
4.9
作者：
Cambray G;Sanchez-Alberola N;Campoy S;Guerin É;Da Re S;González-Zorn B;Ploy MC;Barbé J;Mazel D;Erill I
通讯作者：
Erill I