Test for chemotherapeutic sensitivity of cerebral gliomas: use of colorimetric MTT assay
Test for chemotherapeutic sensitivity of cerebral gliomas: use of colorimetric MTT assay
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脑胶质瘤化疗敏感性测试：使用比色 MTT 测定
作者：
J. Jordan;C. Hand;R. Markowitz;P. Black
本研究旨在评价MTT比色法[3-（4，5-dimethyl-2-thiazolyl）-2，5-diphenyl-2 H tetrazolium bromide]作为体外检测胶质瘤对化疗药物敏感性的一种方法。将8个人神经胶质瘤建立的细胞系接种在96孔组织培养板中，并与10种不同的抗癌剂孵育4天;测试5种不同浓度的每种药物。然后将MTT染料加入到威尔斯孔中，用二甲基亚砜（DMSO）溶解所得甲瓒沉淀。使用对照和实验威尔斯孔的分光光度吸光度（在570 nm处测量）计算细胞毒性指数（CI）。CI值大于50%的生长抑制表明细胞毒性疗效（化疗药物的敏感性）。7个胶质瘤细胞系中有6个（85.7%）在不同浓度下对丝裂霉素C、顺铂和阿霉素高度敏感。7个细胞系中有4个（57.1%）对米托蒽醌和长春碱表现出中等敏感性。7个细胞系中有5个（71.4%）表现出对依托泊苷、博莱霉素、Cosmegen和BCNU的耐药。其中一种检测的细胞系U-138 MG不能产生MTT甲瓒沉淀，因此无法确定该细胞系对化疗药物的敏感性。结果的变异性表明需要一种体外筛选方法来评估临床和实验化疗药物的有效性。MTT试验提供了一种快速筛选抗胶质瘤药物细胞毒性的方法。
SummaryThis study was undertaken to evaluate the colorimetric MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay as a means of testing the sensitivity of gliomas to chemotherapeutic agents in vitro. Eight human glioma established cell lines were plated in 96-well tissue culture plates and incubated for 4 days with 10 different anti-cancer agents; 5 different concentrations of each drug were tested. The MTT dye was then added to the wells, and the resulting formazan precipitate was solubilized with dimethylsulfoxide (DMSO). The spectrophotometric absorbance (measured at 570 nm) of control and experimental wells was used to calculate the cytotoxicity index (CI). Values with a CI greater than 50% growth inhibition indicated cytotoxic efficacy (sensitivity to the chemotherapeutic drug).Six of the seven (85.7%) glioma cell lines were highly sensitive at varying concentrations to mitomycin C, cisplatin, and doxorubicin. Four of the seven (57.1%) cell lines demonstrated intermediate sensitivity to mitoxantrone and vinblastine. Five of the seven (71.4%) cell lines exhibited resistance to etoposide, bleomycin, cosmegen, and BCNU. One of the cell lines tested, U-138MG, failed to produce the MTT formazan precipitate, so that the sensitivity of this cell line to the panel chemotherapeutic drugs could not be determined. The variability of the results indicates the need for an in vitro screening method to evaluate the effectiveness of clinical and experimental chemotherapeutic agents. The MTT assay provides a rapid method of screening antineoplastic agents against gliomas for cytotoxicity.
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影响因子：
2.2
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
11.2
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
10.3
作者：
WEISLOW, OS;KISER, R;BOYD, MR
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