Capabilities and Disadvantages of Combined Matrix-Assisted Laser-Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) and High-Performance Thin-Layer Chromatography (HPTLC): Analysis of Egg Yolk Lipids
Capabilities and Disadvantages of Combined Matrix-Assisted Laser-Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) and High-Performance Thin-Layer Chromatography (HPTLC): Analysis of Egg Yolk Lipids
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基质辅助激光解吸/电离飞行时间质谱 (MALDI-TOF MS) 和高性能薄层色谱 (HPTLC) 组合的功能和缺点：蛋黄脂质分析
DOI：
10.1556/jpc.22.2009.1.7
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
D. Suckau
中科院分区：
文献类型：
--
作者：
B. Fuchs;J. Schiller;R. Süss;A. Nimptsch;M. Schürenberg;D. Suckau
脂类是重要的天然产物，在营养、化妆品、医药等方面必不可少。脂类，特别是磷脂，具有重要的医学价值(一些是具有信使功能的分子)和诊断潜力(例如，人类血液中的脂蛋白)。在各种能够检测完整脂分子的软电离质谱学方法中，基质辅助激光解吸/电离飞行时间质谱仪(MALDI-TOF MS)具有操作简单、灵敏度高、抗污染能力强等优点。此外，MALDI-TOF MS分析固体样品。这使得(与各向同性溶液相比)能够获得空间分辨的质谱图(“质谱学成像”)。然而，为了能够检测所有成分，通常需要将复杂的混合物分离成单独的脂类。以蛋黄中的脂类提取物为例，MALDI-TOF MS可以很容易地与TLC结合，从而能够直接在HPTLC板上检测到微微摩尔量的脂类。这导致了比传统染色程序更高的灵敏度。此外，由于正常相TLC具有很高的空间分辨率，因此不仅可以根据其头基的不同，还可以根据其脂肪酰基组成的不同来区分脂类。最后，也可以直接在HPTLC板上进行MS-MS实验，以提供对相关脂类结构的进一步了解。虽然HPTLC-MALDI耦合可以很容易地建立，但有不同的点需要特别注意。本文将重点介绍矩阵应用、数据获取(包括脂类的稳定性和重复性)以及数据评估等方面的内容。
Lipids are important natural products and essential in nutrition, cosmetic formulations, pharmaceuticals, etc. Lipids and, particularly, phospholipids are of substantial medical interest (some are molecules with messenger function) and of diagnostic potential (for instance, the lipoproteins in human blood). Among the different soft-ionization mass spectrometric methods that enable detection of the intact lipid molecules, matrix-assisted laser-desorption/ioniza-tion time-of-flight mass spectrometry (MALDI-TOF MS) has several advantages, for instance, simple performance, high sensitivity, and robustness against contaminants. Additionally, MALDI-TOF MS analyzes a solid sample. This enables (in contrast with isotropic solutions) acquisition of spatially-resolved mass spectra (‘mass spec-trometric imaging’). However, separation of complex mixtures into the individual lipid classes is normally required to enable detection of all the components. It will be shown with the example of a lipid extract from hens’ egg yolk that MALDI-TOF MS can be easily combined with TLC, enabling detection of as little as picomole amounts of lipids directly on the HPTLC plate. This results in sensitivities higher than those from established staining procedures. Additionally, because of the substantial spatial resolution, lipids separated by normal-phase TLC may not only be differentiated according to differences of their headgroups but also according to differences of their fatty acyl composition. Finally, MS-MS experiments, providing further insights into the structures of the relevant lipids, can be also performed directly on the HPTLC plate. Although the HPTLC-MALDI coupling can be easily established, there are different points to which special attention should be paid. Aspects of matrix application, data acquisition (including the stability of lipids and reproducibility), and data evaluation will be emphasized in this paper.
影响因子：
6.5
作者：
Cynthia J. DeLong;Paul R. S. Baker;M. Samuel;Zheng Cui;Michael J. Thomas
通讯作者：
Cynthia J. DeLong;Paul R. S. Baker;M. Samuel;Zheng Cui;Michael J. Thomas
影响因子：
2.3
作者：
Chaurand, Pierre;Schriver, Kenneth E.;Caprioli, Richard M.
通讯作者：
Caprioli, Richard M.