MicroRNAs expression profile in CCR6(+) regulatory T cells.
MicroRNAs expression profile in CCR6(+) regulatory T cells.
复制标题
CCR6( ) 调节性 T 细胞中的 MicroRNA 表达谱
作者：
Zhao J;Li Y;Hu Y;Chen C;Zhou Y;Tao Y;Guo M;Qin N;Xu L
背景。CCR6+ CD4+调节性T细胞（CCR6+ Tregs）是一种独特的Tregs亚群，在各种免疫疾病中发挥重要作用。最近的证据表明，microRNAs （miRNAs）是免疫细胞功能的重要调节因子。然而，mirna在CCR6+ Tregs功能中的潜在作用在很大程度上仍然未知。在本研究中，我们检测了CCR6+ Tregs中mirna的表达谱。材料与方法。采用微阵列技术检测Balb/c小鼠CCR6+ treg和CCR6- treg中mirna及相关基因的表达谱。使用Keggs通路库分析信号通路。结果。我们发现，与CCR6- Tregs相比，CCR6+ Tregs中有58个mirna显著上调，62个mirna下调，上调幅度高达2倍。此外，通过Keggs通路文库，观察到1391个基因发生了3倍的变化，并富集了20条信号通路。结论。目前的数据显示，CCR6+ Tregs表达了特定的miRNAs模式，这为miRNAs在不同Tregs亚群的生物学功能中的作用提供了深入的见解。
Backgroud. CCR6+ CD4+ regulatory T cells (CCR6+ Tregs), a distinct Tregs subset, played an important role in various immune diseases. Recent evidence showed that microRNAs (miRNAs) are vital regulators in the function of immune cells. However, the potential role of miRNAs in the function of CCR6+ Tregs remains largely unknown. In this study, we detected the expression profile of miRNAs in CCR6+ Tregs. Materials and Methods. The expression profile of miRNAs as well as genes in CCR6+ Tregs or CCR6- Tregs from Balb/c mice were detected by microarray. The signaling pathways were analyzed using the Keggs pathway library. Results. We found that there were 58 miRNAs significantly upregulated and 62 downregulated up to 2 fold in CCR6+ Tregs compared with CCR6- Tregs. Moreover, 1,391 genes were observed with 3 fold change and 20 signaling pathways were enriched using the Keggs pathway library. Conclusion. The present data showed CCR6+ Tregs expressed specific miRNAs pattern, which provides insight into the role of miRNAs in the biological function of distinct Tregs subsets.
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DOI：
10.1084/jem.20091021
发表时间：
2010-03-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Rivino L;Gruarin P;Häringer B;Steinfelder S;Lozza L;Steckel B;Weick A;Sugliano E;Jarrossay D;Kühl AA;Loddenkemper C;Abrignani S;Sallusto F;Lanzavecchia A;Geginat J
通讯作者：
Geginat J
影响因子：
3.7
作者：
Sommers CL;Rouquette-Jazdanian AK;Robles AI;Kortum RL;Merrill RK;Li W;Nath N;Wohlfert E;Sixt KM;Belkaid Y;Samelson LE
通讯作者：
Samelson LE
影响因子：
8.6
作者：
Xu, Lin;Xu, Wei;Xiong, Sidong
通讯作者：
Xiong, Sidong
DOI：
10.4049/jimmunol.1001156
发表时间：
2010-09-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kitamura K;Farber JM;Kelsall BL
通讯作者：
Kelsall BL
影响因子：
2.1
作者：
Johanson, Timothy M.;Skinner, Jarrod P. J.;Chong, Mark M. W.
通讯作者：
Chong, Mark M. W.