Characterization of the Hsp110/SSE gene family response to hyperosmolality and other stresses.
Characterization of the Hsp110/SSE gene family response to hyperosmolality and other stresses.
复制标题
Hsp110/SSE 基因家族对高渗透压和其他应激反应的表征。
DOI：
10.1152/ajprenal.1998.274.6.f1054
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Gullans,SR
中科院分区：
文献类型：
--
作者：
Santos,BC;Chevaile,A;Kojima,R;Gullans,SR
Hsp 110、Osp 94和Hsp 70 RY是最近描述的（热和渗透）应激蛋白的Hsp 110/SSE亚家族的成员，其成员在结构上与Hsp 70/BiP基因超家族相关。迄今为止，很少有人知道这个基因家族的反应，以强调在体外或体内。在这项研究中，mRNA表达的分析表明，Hsp 110和Osp 94，热休克，高渗氯化钠，镉，而低pH值对Osp 94有抑制作用，诱导在肾内髓集合管（mIMCD 3）上皮细胞的Hsp 70。H2 O2诱导Hsp 110和Hsp 70的表达，同时降低Osp 94的表达。衣霉素、高渗尿素和肿瘤坏死因子-α无影响。Hsp 70 RY只对氯化镉有反应。此外，Hsp 110和Osp 94的表达增强后，诱导Hsp 70和蛋白质合成的抑制放线菌酮抑制。RT-PCR分析表明Hsp 110、Osp 94和Hsp 70 RY在小鼠组织中广泛表达。在小鼠肾脏中，有一个皮质髓质梯度的表达Hsp 110，Osp 94，Hsp 70 RY，和Hsp 70，但没有Hsc 70或BiP。此外，脱水增加了Hsp 110和Osp 94的内髓表达。对mIMCD 3细胞应激耐受性的分析表明，热休克和高渗氯化钠应激是交叉耐受应激，这表明高渗透压是哺乳动物肾脏热休克的生理相关性。因此，Hsp 110和Osp 94表现为热休克蛋白，尽管它们的调节与Hsp 70不同。
Hsp110, Osp94, and Hsp70RY are members of the recently described Hsp110/SSE subfamily of (heat and osmotic) stress proteins whose members are structurally related to the Hsp70/BiP gene superfamily. To date, little is known about the response of this gene family to stresses in vitro or in vivo. In this study, an analysis of mRNA expression showed that Hsp110 and Osp94, like Hsp70, are induced in renal murine inner medullary collecting duct (mIMCD3) epithelial cells by heat shock, hyperosmotic NaCl, and cadmium, whereas low pH had a suppressive effect on Osp94. H2O2decreased expression of Osp94 while inducing levels of Hsp110 and Hsp70 message. Tunicamycin, hypertonic urea, and tumor necrosis factor-α had no effects. Hsp70RY was responsive exclusively to cadmium chloride. Moreover, enhanced expression of Hsp110 and Osp94 was subsequent to induction of Hsp70 and was suppressed by inhibition of protein synthesis by cycloheximide. RT-PCR analysis showed Hsp110, Osp94, and Hsp70RY are ubiquitously expressed in mouse tissues. In murine kidney, there was a corticomedullary gradient of expression of Hsp110, Osp94, Hsp70RY, and Hsp70 but not Hsc70 or BiP. Furthermore, dehydration increased inner medullary expression of Hsp110 and Osp94. An analysis of stress tolerance in mIMCD3 cells showed that heat shock and hyperosmotic NaCl stress are cross-tolerant stresses, suggesting hyperosmolality is a physiological correlate of heat shock in mammalian kidney. Thus Hsp110 and Osp94 behave as heat shock proteins, although they are regulated differently than Hsp70.
登录
查看更多内容
影响因子：
56.9
作者：
BECKMANN, RP;MIZZEN, LA;WELCH, WJ
通讯作者：
WELCH, WJ
影响因子：
--
作者：
RAUCHMAN, MI;NIGAM, SK;GULLANS, SR
通讯作者：
GULLANS, SR
渗透应激蛋白 94 (Osp94)
影响因子：
4.8
作者：
R. Kojima;J. Randall;B. Brenner;S. Gullans
通讯作者：
S. Gullans
小鼠内髓集合管细胞高渗应激诱导分子伴侣。
DOI：
10.1152/ajprenal.1997.273.1.f9
发表时间：
1997
期刊：
The American journal of physiology.
影响因子：
--
作者：
Rauchman,MI;Pullman,J;Gullans,SR
通讯作者：
Gullans,SR
先前的热应激可增强 ATP 耗尽后肾上皮细胞的存活率。
影响因子：
--
作者：
Y. Wang;S. Borkan
通讯作者：
S. Borkan