Formation of versus Recruitment to RNA-Rich Condensates: Controlling Effects Exerted by Peptide Side Chain Identity.
Formation of versus Recruitment to RNA-Rich Condensates: Controlling Effects Exerted by Peptide Side Chain Identity.
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DOI：
10.1021/jacs.2c02222
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发表时间：
2022-06-15
影响因子：
15
通讯作者：
Gellman, Samuel H.
中科院分区：
文献类型：
--
作者：
Niu, Jiani;Qiu, Cindy;Abbott, Nicholas L.;Gellman, Samuel H.
液-液相分离（LLPS）是具有不同组成的连续液相的自发形成，其在化学系统中早已为人所知，并且最近被认为是细胞生物学的普遍特征。我们描述了一个系统，涉及生物相关的组件，合成肽和总酵母RNA，使我们能够探索相分离的基础因素。阳离子肽和阴离子RNA之间的库仑互补性是必要的，但不足以在我们的系统中形成凝聚相。除了净正电荷之外，肽必须存在适当类型的阳离子部分。胍鎓基团，如发现在Arg侧链，支持相分离，但铵基团，如发现在Lys侧链，或二甲基胍鎓基团，如发现在后修饰的Arg侧链，不支持在我们的系统中的相分离。然而，不支持通过与RNA相互作用的相分离的阳离子基团仍然能够募集到凝聚相，这表明控制凝聚相形成的力的网络可以不同于控制募集到这种相的力的网络。介绍了一种基于含氟添加剂和19 F NMR测定凝聚相中组分浓度的新方法。
Liquid-liquid phase separation (LLPS), which is the spontaneous formation of contiguous liquid phases with distinct compositions, has been long known in chemical systems and more recently recognized as a ubiquitous feature of cell biology. We describe a system involving biologically relevant components, synthetic peptides and total yeast RNA, that has enabled us to explore factors that underlie phase separation. Coulombic complementarity between a cationic peptide and anionic RNA is necessary but not sufficient for formation of a condensed phase in our system. In addition to a net positive charge, the peptide must present the proper type of cationic moiety. Guanidinium groups, as found in the Arg side chain, support phase separation, but ammonium groups, as found in the Lys side chain, or dimethylguanidinium groups, as found in post-translationally modified Arg side chains, do not support phase separation in our system. However, the cationic groups that do not support phase separation via interaction with RNA can nevertheless enable recruitment to a condensed phase, which reveals that the network of forces governing condensed phase formation can differ from the network of forces governing recruitment to such a phase. We introduce a new method for measuring the concentrations of components in condensed phases based on fluorine-containing additives and 19F NMR.
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影响因子：
16.6
作者：
Bramham JE;Golovanov AP
通讯作者：
Golovanov AP
DOI：
10.1073/pnas.1907849116
发表时间：
2019-09-24
影响因子：
11.1
作者：
Ghosh, Archishman;Mazarakos, Konstantinos;Zhou, Huan-Xiang
通讯作者：
Zhou, Huan-Xiang
影响因子：
21.8
作者：
Aumiller, William M., Jr.;Keating, Christine D.
通讯作者：
Keating, Christine D.
影响因子：
--
作者：
Frigyes, D;Alber, F;Carloni, P
通讯作者：
Carloni, P
影响因子：
19
作者：
Boeynaems S;Alberti S;Fawzi NL;Mittag T;Polymenidou M;Rousseau F;Schymkowitz J;Shorter J;Wolozin B;Van Den Bosch L;Tompa P;Fuxreiter M
通讯作者：
Fuxreiter M