DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device.
DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device.
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DOI：
10.1039/b921417h
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发表时间：
2010-05-07
期刊：
影响因子：
6.1
通讯作者：
Huang X
中科院分区：
文献类型：
--
作者：
Chen YJ;Roller EE;Huang X
我们报告了一种新的DNA测序技术的概念证明，称为变性测序（SBD）。SBD基于在固定于固体表面上的扩增靶模板上进行的桑格测序反应，然后使这些桑格片段变性。随着这些荧光标记的片段依次变性，在流动池中监测对应于四种碱基类型的四个通道中的荧光强度。具有微流体流动池的测序仪器已被定制设计成集成自动化流体、温度控制和荧光成像。用该系统测定了几种合成寡核苷酸的变性谱，我们的数据证明了SBD对短DNA测序的能力。SBD是一种简单快速的方法，有可能提高大规模基因组重测序的速度和成本。
We report the proof of concept of a novel DNA sequencing technology called sequencing by denaturation (SBD). SBD is based on the Sanger sequencing reaction performed on amplified target templates immobilized on a solid surface followed by the denaturation of these Sanger fragments. As these fluorescently labeled fragments denature sequentially, the fluorescence intensities in the four channels corresponding to the four base types are monitored in a flow cell. A sequencing instrument with a microfluidic flowcell has been custom-designed to integrate automated fluidics, temperature control, and fluorescence imaging. The denaturation profiles of several synthetic oligonucleotides were measured with this system and our data demonstrated the ability to sequence short DNA by SBD. SBD is a simple and fast method with the potential to improve the speed and cost of large-scale genome re-sequencing.
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影响因子：
46.9
作者：
Ball, Madeleine P.;Li, Jin Billy;Gao, Yuan;Lee, Je-Hyuk;LeProust, Emily M.;Park, In-Hyun;Xie, Bin;Daley, George Q.;Church, George M.
通讯作者：
Church, George M.
影响因子：
46.9
作者：
Deng, Jie;Shoemaker, Robert;Xie, Bin;Gore, Athurva;LeProust, Emily M.;Antosiewicz-Bourget, Jessica;Egli, Dieter;Maherali, Nimet;Park, In-Hyun;Yu, Junying;Daley, George Q.;Eggan, Kevin;Hochedlinger, Konrad;Thomson, James;Wang, Wei;Gao, Yuan;Zhang, Kun
通讯作者：
Zhang, Kun
DOI：
10.1073/pnas.0702165104
发表时间：
2007-05-29
影响因子：
11.1
作者：
Dahl, Fredrik;Stenberg, Johan;Ji, Hanlee
通讯作者：
Ji, Hanlee
影响因子：
56.9
作者：
Shendure, J;Porreca, GJ;Church, GM
通讯作者：
Church, GM
影响因子：
2.9
作者：
Chen, Ying-Ja;Huang, Xiaohua
通讯作者：
Huang, Xiaohua