Fount, fate, features, and function of renal erythropoietin-producing cells.
Fount, fate, features, and function of renal erythropoietin-producing cells.
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DOI：
10.1007/s00424-022-02714-7
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发表时间：
2022-08
影响因子：
4.5
通讯作者：
Wenger, Roland H.
中科院分区：
文献类型：
--
作者：
Dahl, Sophie L.;Bapst, Andreas M.;Khodo, Stellor Nlandu;Scholz, Carsten C.;Wenger, Roland H.
肾促红细胞生成素（Epo）产生（REP）细胞是一种罕见且尚未完全了解的细胞类型。REP细胞是成纤维细胞样细胞，位于皮质髓质边缘区域的血管和小管附近。REP细胞中的Epo mRNA以明显的“开-关”模式产生，在暴露于缺氧时显示瞬时转录爆发。与“普通”成纤维细胞相反，REP细胞不离体增殖，停止产生Epo，并且在永生化和延长的体外培养后失去其特性，这与慢性肾病中组织重塑期间REP细胞增殖后Epo产生的丧失一致。由于Epo蛋白在肾组织中通常检测不到，并且Epo mRNA仅在缺氧条件下瞬时诱导，因此已开发出转基因小鼠模型来永久标记REP细胞前体、活性Epo生产者和非活性后代。未来的肾间质室的单细胞分析将确定标记REP细胞的新特征标记物，这将为这种独特的细胞类型中Epo表达的调节提供新的见解。
Renal erythropoietin (Epo)-producing (REP) cells represent a rare and incompletely understood cell type. REP cells are fibroblast-like cells located in close proximity to blood vessels and tubules of the corticomedullary border region. Epo mRNA in REP cells is produced in a pronounced “on–off” mode, showing transient transcriptional bursts upon exposure to hypoxia. In contrast to “ordinary” fibroblasts, REP cells do not proliferate ex vivo, cease to produce Epo, and lose their identity following immortalization and prolonged in vitro culture, consistent with the loss of Epo production following REP cell proliferation during tissue remodelling in chronic kidney disease. Because Epo protein is usually not detectable in kidney tissue, and Epo mRNA is only transiently induced under hypoxic conditions, transgenic mouse models have been developed to permanently label REP cell precursors, active Epo producers, and inactive descendants. Future single-cell analyses of the renal stromal compartment will identify novel characteristic markers of tagged REP cells, which will provide novel insights into the regulation of Epo expression in this unique cell type.
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影响因子：
30.8
作者：
Chambers, John C.;Zhang, Weihua;Li, Yun;Sehmi, Joban;Wass, Mark N.;Zabaneh, Delilah;Hoggart, Clive;Bayele, Henry;McCarthy, Mark I.;Peltonen, Leena;Freimer, Nelson B.;Srai, Surjit K.;Maxwell, Patrick H.;Sternberg, Michael J. E.;Ruokonen, Aimo;Abecasis, Goncalo;Jarvelin, Marjo-Riitta;Scott, James;Elliott, Paul;Kooner, Jaspal S.
通讯作者：
Kooner, Jaspal S.
影响因子：
5.6
作者：
Bapst, Andreas M.;Knopfel, Thomas;Nolan, Karen A.;Imeri, Faik;Schuh, Claus D.;Hall, Andrew M.;Guo, Jia;Katschinski, Doerthe M.;Wenger, Roland H.
通讯作者：
Wenger, Roland H.
影响因子：
10.1
作者：
Corre T;Ponte B;Pivin E;Pruijm M;Ackermann D;Ehret G;Spanaus K;Bochud M;Wenger RH
通讯作者：
Wenger RH
影响因子：
6.3
作者：
Dahl, Sophie L.;Pfundstein, Svende;Hunkeler, Rico;Dong, Xingtong;Knoepfel, Thomas;Spielmann, Patrick;Scholz, Carsten C.;Nolan, Karen A.;Wenger, Roland H.
通讯作者：
Wenger, Roland H.
影响因子：
3.4
作者：
Aboushwareb, Tamer;Egydio, Fernanda;Yoo, James J.
通讯作者：
Yoo, James J.