A novel technique for monitoring the development of bacterial biofilms in human periodontal pockets.
A novel technique for monitoring the development of bacterial biofilms in human periodontal pockets.
复制标题
一种监测人类牙周袋中细菌生物膜发育的新技术。
DOI：
10.1111/j.1574-6968.2000.tb09324.x
复制
发表时间：
2000
影响因子：
2.1
通讯作者：
J. Bernimoulin
中科院分区：
文献类型：
--
作者：
J. Wecke;T. Kersten;Kasimierz Madela;A. Moter;U. Göbel;A. Friedmann;J. Bernimoulin
A new technique is presented for analyzing subgingival bacterial plaque. Different materials (polytetrafluoroethylene, gold, dentin) kept for several days in periodontal pockets of patients suffering from periodontitis were analyzed by electron microscopy and fluorescence in situ hybridization (FISH). Those parts of the carriers extending into the deepest zone of the pockets were predominantly colonized by spirochetes and Gram-negative bacteria whereas those segments in contact with a shallower region were colonized by streptococci. Independent of the material used, the bacterial colonization of the carriers appears to be similar. FISH using eubacteria- and species-specific oligonucleotides on semi-thin cross-sections of the carriers in combination with confocal laser scanning microscopy allowed detailed analysis of the architecture of biofilms and identification of putative periodontal pathogens with single cell resolution.
影响因子：
6.7
作者：
Socransky, SS;Haffajee, AD;Kent, RL
通讯作者：
Kent, RL
口腔生物膜的生理稳态和应激反应。
影响因子：
--
作者：
Burne,RA;QuiveyJr,RG;Marquis,RE
通讯作者：
Marquis,RE
影响因子：
4.3
作者：
Page,RC;Altman,LC;Ebersole,JL;Vandesteen,GE;Dahlberg,WH;Williams,BL;Osterberg,SK
通讯作者：
Osterberg,SK