Comparative transcriptome analysis of obligately asexual and cyclically sexual rotifers reveals genes with putative functions in sexual reproduction, dormancy, and asexual egg production.
Comparative transcriptome analysis of obligately asexual and cyclically sexual rotifers reveals genes with putative functions in sexual reproduction, dormancy, and asexual egg production.
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DOI：
10.1186/1471-2164-14-412
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发表时间：
2013-06-19
期刊：
影响因子：
4.4
通讯作者：
Logsdon JM Jr
中科院分区：
文献类型：
--
作者：
Hanson SJ;Stelzer CP;Welch DB;Logsdon JM Jr
有性繁殖是一个被广泛研究的生物学过程，因为它对真核生物的遗传学、进化和生态学至关重要。尽管对这一主题进行了数十年的研究，但还没有人接受全面的解释来解释其流行和持续存在的自然界背后的进化力量。单角类轮虫繁殖速度快，与古老的无性轮虫关系密切，为有性生殖进化的实验研究提供了一个有用的系统。然而，人们对任何轮虫物种的性行为的分子基础知之甚少。我们构建了花瓣臂尾轮虫专性孤雌生殖(OP)和周期孤雌生殖(CP)品系的mRNA-seq文库，以鉴定这两种繁殖模式的特异基因。我们的差异表达分析确定了受体在信号通路中可能扮演的角色，这些信号通路负责从无性生殖向有性生殖的转变。复制的细胞周期调控基因CDC20的特定拷贝和组蛋白H2A的特定拷贝的差异表达表明，这种重复可能是单胞体生殖模式切换所需的表型可塑性的基础。我们进一步确定了与休眠卵子形成有关的基因的差异表达，这一过程仅与该物种的性别有关。最后，我们鉴定了蓖麻瓢虫的转录本，这些转录本与花苞瓢虫CP株中高表达的基因具有显著的序列相似性。我们对花瓣假单胞虫兼性种群和完全无性种群的全球基因表达差异的分析为了解轮虫有性繁殖的分子本质提供了洞察力。此外，我们的结果提供了对蜈蚣专性无性的进化的洞察力，并为使用单性体作为研究有性繁殖进化的模型系统提供了重要的指标。
Sexual reproduction is a widely studied biological process because it is critically important to the genetics, evolution, and ecology of eukaryotes. Despite decades of study on this topic, no comprehensive explanation has been accepted that explains the evolutionary forces underlying its prevalence and persistence in nature. Monogonont rotifers offer a useful system for experimental studies relating to the evolution of sexual reproduction due to their rapid reproductive rate and close relationship to the putatively ancient asexual bdelloid rotifers. However, little is known about the molecular underpinnings of sex in any rotifer species. We generated mRNA-seq libraries for obligate parthenogenetic (OP) and cyclical parthenogenetic (CP) strains of the monogonont rotifer, Brachionus calyciflorus, to identify genes specific to both modes of reproduction. Our differential expression analysis identified receptors with putative roles in signaling pathways responsible for the transition from asexual to sexual reproduction. Differential expression of a specific copy of the duplicated cell cycle regulatory gene CDC20 and specific copies of histone H2A suggest that such duplications may underlie the phenotypic plasticity required for reproductive mode switch in monogononts. We further identified differential expression of genes involved in the formation of resting eggs, a process linked exclusively to sex in this species. Finally, we identified transcripts from the bdelloid rotifer Adineta ricciae that have significant sequence similarity to genes with higher expression in CP strains of B. calyciflorus. Our analysis of global gene expression differences between facultatively sexual and exclusively asexual populations of B. calyciflorus provides insights into the molecular nature of sexual reproduction in rotifers. Furthermore, our results offer insight into the evolution of obligate asexuality in bdelloid rotifers and provide indicators important for the use of monogononts as a model system for investigating the evolution of sexual reproduction.
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影响因子：
16.8
作者：
Buschbeck, Marcus;Uribesalgo, Iris;Di Croce, Luciano
通讯作者：
Di Croce, Luciano
DOI：
10.1016/j.cbpb.2004.10.019
发表时间：
2004-12-01
影响因子：
2.3
作者：
Caprioli, M;Katholm, AK;Santo, N
通讯作者：
Santo, N
DOI：
10.1007/978-3-642-12422-8_7
发表时间：
2010-01-01
期刊：
DORMANCY AND RESISTANCE IN HARSH ENVIRONMENTS
影响因子：
--
作者：
Denekamp, Nadav Y.;Suga, Koushirou;Lubzens, Esther
通讯作者：
Lubzens, Esther
影响因子：
9.8
作者：
Bridgham, Jamie T.;Eick, Geeta N.;Thornton, Joseph W.
通讯作者：
Thornton, Joseph W.
影响因子：
9.2
作者：
Erkut, Cihan;Penkov, Sider;Kurzchalia, Teymuras V.
通讯作者：
Kurzchalia, Teymuras V.