A Noninvasive Genetic/Pharmacologic Strategy for Visualizing Cell Morphology and Clonal Relationships in the Mouse
A Noninvasive Genetic/Pharmacologic Strategy for Visualizing Cell Morphology and Clonal Relationships in the Mouse
复制标题
用于可视化小鼠细胞形态和克隆关系的非侵入性遗传/药理学策略
DOI：
10.1523/jneurosci.23-06-02314.2003
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
J. Nathans
中科院分区：
文献类型：
--
作者：
T. Badea;Yanshu Wang;J. Nathans
细胞形态学分析是神经元分类的最常用方法。随着基因工程小鼠的使用增加，越来越需要能够选择性地可视化指定神经元子集的形态的方法。需要这种能力来定义细胞形态表型和以非侵入性方式标记细胞用于谱系研究。为此，我们描述了一种基于Cre-estrogen receptor（ER）融合蛋白的二分遗传系统，该融合蛋白通过位点特异性重组不可逆地激活质膜结合的碱性磷酸酶报告基因。由于基因重排的效率和时间是可控的，因此在发育过程中的任何时候都可以从CreER表达细胞组中产生标记细胞的稀疏子集。碱性磷酸酶活性的组织化学可视化显示神经元形态，所有过程具有强烈和均匀的标记。
Analysis of cellular morphology is the most general approach to neuronal classification. With the increased use of genetically engineered mice, there is a growing need for methods that can selectively visualize the morphologies of specified subsets of neurons. This capability is needed both to define cell morphologic phenotypes and to mark cells in a noninvasive manner for lineage studies. To this end, we describe a bipartite genetic system based on a Cre-estrogen receptor (ER) fusion protein that irreversibly activates a plasma membrane-bound alkaline phosphatase reporter gene by site-specific recombination. Because the efficiency and timing of gene rearrangement is controlled pharmacologically, a sparse subset of labeled cells can be generated from the set of CreER-expressing cells at any time during development. Histochemical visualization of alkaline phosphatase activity reveals neuronal morphology with strong and uniform labeling of all processes.
影响因子：
4.6
作者：
H. Wichterle;D. Turnbull;S. Nery;G. Fishell;A. Álvarez-Buylla
通讯作者：
H. Wichterle;D. Turnbull;S. Nery;G. Fishell;A. Álvarez-Buylla
影响因子：
10.5
作者：
FRIEDRICH, G;SORIANO, P
通讯作者：
SORIANO, P
影响因子：
2.7
作者：
Hayashi, S;McMahon, AP
通讯作者：
McMahon, AP