CD66 identifies a neutrophil-specific epitope within the hematopoietic system that is expressed by members of the carcinoembryonic antigen family of adhesion molecules.-猫眼课题宝

CD66 识别造血系统内的中性粒细胞特异性表位，该表位由粘附分子的癌胚抗原家族成员表达。

DOI：

10.1182/blood.v78.1.63.bloodjournal78163

发表时间：

1991

期刊：

Blood

影响因子：

20.3

通讯作者：

中科院分区：

医学1区

文献类型：

作者：

Suzanne M. Watt;G. Sala;Trang Hoang;David J. Gilmore;Fritz Grunert;G. Nagel;SJ Murdoch;E. Tchilian;ES Lennox;Herman Waldmann

第四届白细胞培养会议的初步结果已将单克隆抗体（MoAb）YTH 71.3.2归类为CD 66。另外两种单克隆抗体YPC 2/12.1和CE 6/2D3.1具有共同的细胞特异性，与中性粒细胞系和结肠上皮细胞反应。YTH 71.3.2和CE 6/2D3.1 MoAb均识别与YPC 2/12.1鉴定的表位不同的类似的CD 66限定的表位。通过Western印迹，这些抗体与来自不同谱系细胞的不同分子种类反应。这些抗体识别来自结肠腺癌的半纯化癌胚抗原（CEA）制剂中存在的50- 55-Kd、80- 100-Kd和130- 200-Kd组分和来自HL-60细胞的90- 130-Kd分子。对于结肠细胞系，LS 174 T、YPC 2/12.1以相等强度染色160至200 Kd和90至130 Kd的扩散带，而CE 6/2D3.1和YTH 71.3.2的结合偏向于较低分子量的分子组。值得注意的是，所有三种抗体都识别CEA相关分子。使用用CEA、NCA（NCA-50/90）和CGM 6（NCA-95）cDNA转染的HeLa细胞的限定分析显示，三种MoAb在不同程度上鉴定CEA。虽然YTH 71.3.2和CE 6/2D3.1也与NCA-50/90结合，但YPC 2/12.1识别由NCA-50/90和NCA-95分子种类表达的表位。

Preliminary results from the IVth Leucocyte Culture Conference have classified the monoclonal antibody (MoAb), YTH 71.3.2, as CD66. Two other MoAbs, YPC 2/12.1 and CE6/2D3.1, share a common cellular specificity, reacting with cells of the neutrophil series and colonic epithelium. The YTH 71.3.2 and CE6/2D3.1 MoAbs both recognize a similar CD66 defined epitope that is distinct from that identified by YPC 2/12.1. By Western blotting, these antibodies react with different molecular species from cells of different lineages. The antibodies identify 50- to 55-Kd, 80- to 100-Kd, and 130- to 200-Kd components present in a semi-purified carcinoembryonic antigen (CEA) preparation from colonic adenocarcinomas and a 90- to 130-Kd molecule from HL-60 cells. With the colonic cell line, LS174T, YPC2/12.1 stains diffuse bands of 160 to 200 Kd and 90 to 130 Kd with equal intensity, whereas the binding of CE6/2D3.1 and YTH 71.3.2 is biased toward the lower molecular weight set of molecules. Remarkably, all three antibodies recognize CEA-related molecules. Defined analyses using HeLa cells transfected with CEA, NCA(NCA-50/90), and CGM6(NCA-95) cDNAs show that the three MoAbs identify CEA to varying degrees. While YTH 71.3.2 and CE6/2D3.1 also bind to NCA-50/90, YPC 2/12.1 recognizes an epitope expressed by both the NCA-50/90 and NCA-95 molecular species.